Ras分子信号蛋白调控睾丸间质细胞Cox7a2蛋白表达及共定位影响研究  

Efect of Ras signal regulating Cox7a2 expression and its sub-cellular location in testicular interstitial cell

作　　者：陈亮[1] 徐阳[1] 左文莉[1] 杨慧霞[1] 廖秦平[1] 辛钟成[2] 郭应禄[2] 

机构地区：[1]北京大学第一医院妇产科生殖与遗传医疗中心,北京100034 [2]北京大学第一医院男科中心,北京100009

出　　处：《北京大学学报（医学版）》2012年第4期507-510,共4页Journal of Peking University:Health Sciences

基　　金：国家自然科学基金(30901492);中国国家博士后科学基金第47批面上项目(20100470511)资助~~

摘　　要：目的:研究Cox7a2与Ras分子蛋白的共定位特点,探讨迟发性性腺功能障碍(late onset hypogonadism,LOH)的分子信号调控机制。方法:构建Cox7a2及Ras正义及点突变体荧光表达载体,鉴定后转染细胞,荧光显微镜观察Cox7a2及Ras蛋白的共定位特点。结果:Cox7a2的线粒体定位能被Wt-Ras所干扰,N17-Ras突变体转染细胞,Cox7a2恢复线粒体定位,提示Cox7a2和N17-Ras之间可能存在相互作用。结论:Cox7a2对睾酮合成的调控可能与Ras相关信号通路有关,Ras可能是调控靶点之一。Objective：To investigate the co-sub-cellular-location of CoxTa2 and Ras. Methods： Ras and its mutant plasmid were cloned by RT-PCR and sequence analysis. Cox7a2-pEYFP-N1, Ras-pEYFP- N1 and N17-Ras-pEYFP-N1 fluorescent protein vectors were constructed and transfected into TM3 cells. Results： Cox7a2 was located in the mitochondria, but its location was changed by the expression of Ras. When the dominat negative ras was expressed in the cells, the Cox7a2 located into the mitochondria again. Conclusion： CoxTa2 mediated testosterone production, which might be at least in part related with the Ras signaling pathway. Ras may be the regulating target and further investigation is needed to make it clear.

关 键 词：性腺功能减退症 LEYDIG细胞 Cox7a2蛋白 RAS蛋白 睾酮 

分 类 号：R698[医药卫生—泌尿科学]