非培养法检测阴道微生态的进展  

Research Progress of Detecting Vagina Microecology by Cultivation-Independent Methods

作　　者：陈晴[1,2] 樊尚荣[1] 

机构地区：[1]北京大学深圳医院妇产科,广东深圳5180361 [2]汕头大学医学院,广东汕头515041

出　　处：《医学综述》2012年第15期2467-2470,共4页Medical Recapitulate

摘　　要：女性阴道内寄生着多种微生物,鉴定阴道中存在的微生物有助于了解其在健康和疾病时的作用,但是许多微生物难以通过培养将其分离鉴定。近年来,一系列分子生物学的方法,如基于16SrRNA基因聚合酶链反应扩增法等用于研究阴道微生态并取得了较大的进展。利用分子生物学的非培养法检测女性阴道微生物发现,不论在健康女性还是细菌性阴道病(BV)患者中惰性乳杆菌是普遍存在的乳杆菌;还发现许多新的厌氧菌与BV高度相关,如阴道阿托波菌、阴道加德纳菌、BVAB1等。The human vagina hosts many microbes. Identifying all kinds of microbes in vagina will help to better understand their roles in heath and disease. However,many microbes are difficult to be identified by cul- tivation-dependent methods. In recent years, a series of molecular approaches, such as PCR amplification of 16S rRNA gene,are used to study the vagina microecology and have made considerable progress. The use of molecular, cultivation-independent methods have revealed： lactobacillus inert is the ubiquitous nature in women with or without bacterial vaginosis （BV）; some novel anaerobes are strongly associated with BV, such as Atopobium vaginae, Gardnerella vaginalis , BVAB1 and so on.

关 键 词：非培养法 阴道 微生态 

分 类 号：R711[医药卫生—妇产科学]