一次性力竭运动后心肌核转录因子Kappa B的变化及其在运动性心肌微损伤发生中的作用  

Changes of Myocardial Nuclear Transcription Factor Kappa B and It's Role in Athletic Myocardial Micro-damage after One-time Exhaustive Exercise

作　　者：冀云肖[1] 常芸[1] 

机构地区：[1]国家体育总局体育科学研究所,北京体育馆路11号北京100061

出　　处：《体育科研》2012年第4期25-29,共5页Sport Science Research

基　　金：国家体育总局体育科学研究所基本科研业务经费(10-01)

摘　　要：目的:研究一次性力竭运动后大鼠心肌NF-κB在基因与蛋白水平的分布及变化规律。方法:100只健康成年雄性SD大鼠,随机分为一次性力竭游泳运动组及安静对照组。应用RT-PCR和免疫荧光组化技术,从mRNA及蛋白水平研究大鼠心肌NF-κBp65的在力竭运动后不同时相的分布及表达变化。结果:对照组NF-κB主要分布于细胞质、细胞膜及血管内膜,偶见于细胞核内,力竭运动后NF-κB主要分布于细胞核、细胞膜和血管内膜。一次性力竭后6 h左心室NF-κB p65蛋白含量显著高于对照组、12 h组、24 h组(P<0.05);运动后即刻室间隔NF-κB p65蛋白含量显著高于对照组(P<0.05),非常显著高于12 h组、24 h(P<0.01),运动后6 h非常显著高于12 h组(P<0.01);运动后即刻右心室NF-κBp65蛋白含量即显著高于安静对照组、24 h组(P<0.05),运动后6 h组显著高于安静对照组(P<0.05)。一次性力竭运动后即刻心肌总的NF-κBp65蛋白含量显著高于对照组(P<0.05),6 h组非常显著地高于对照组水平(P<0.01),运动后12 h组显著低于即刻组和6 h组。一次性力竭即刻NF-κBp65mRNA含量显著高于一次性力竭24 h组(P<0.05);6 h显著高于对照组、24 h组(P<0.05);12 h显著高于24 h组(P<0.05)。结论:一次力竭运动后不同时相心肌NF-κBmRNA和蛋白表达增加,可介导一系列炎症反应对细胞造成损伤,构成运动性心肌微损伤中发生机制之一。一次力竭运动后心脏各部位改变存有一定差异,其中室间隔及右心室NF-κB蛋白含量升高速度快,以室间隔NF-κB蛋白峰值水平最高,且右心室NF-κB蛋白改变恢复最慢。Objective To study the distribution and variation laws of rat heart muscle NF-｜êB in gene and protein level after one-time exhaustive exercise.Method 100 healthy adult male SD rats were divided randomly into one-time exhaustive swimming group and control group.RT-PCR and immunofluorescence technology were adopted to study distribution and expression variations of rat heart muscle NF-｜êB p65 at the different phases after exhaustive exercise from mRNA and protein level.Result In control group,NF-｜êB mainly distributed in cell plasma,cell membrane and tunic intima,sometimes in cell nucleus.After exhaustive exercise,NF-｜êB mainly distributed in cell plasma,cell membrane and tunic intima and cell nucleus.6 hours after exhaustive exercise,NF-｜êB p65 protein level of left ventricle is obviously higher than those of the control group,12h group and 24h group（P〈0.05）.The NF-｜êB p65 protein level of ventricular septum immediately after the exercise was significantly higher than that of the control group（P〈0.05） and was much higher than the 12h group and 24h group（P〈0.01）.It was much higher than that of the 12h group 6 hours after the exercise.NF-｜êB p65 protein level of the right ventricle was distinctly higher than those of the control group and 24h group（P〈0.05） and this level of the 6h group was evidently higher than that of the control group（P〈0.05）.The total NF-｜êB p65 protein level of heart muscle immediately after the one-time exhaustive exercise was significantly higher than that of the control group（P〈0.05）.This level of the 6h group was much higher than that of the control group（P〈0.05）.And the level of the 12h group was evidently lower than those of the immediate group and 6h group.NF-｜êB p65mRNA level immediately after the one-time exhaustive exercise was clearly higher than that of the 24h group in one-time exhaustive exercise（P〈0.05）.The level of the 6h group was obviously higher than those of the control group and 24h group（P〈0.05�

关 键 词：力竭运动 核转录因子KappaB(NF-κB) 心肌微损伤 

分 类 号：G804.5[文化科学—运动人体科学]