快中子诱发U251神经胶质瘤细胞的生物效应研究  

A biological effectiveness study on U251 induced by fast neutrons

作　　者：杨磊[1] 王潇[1] 孔福全[1] 隋丽[1] 崔素珍[1] 郑洁莹[1] 马南茹[1] 赵葵[1] 

机构地区：[1]中国原子能科学研究院,北京102413

出　　处：《中国工程科学》2012年第8期91-95,共5页Strategic Study of CAE

基　　金：国家自然科学基金项目(11079054)

摘　　要：目的:以14 MeV快中子照射体外培养的U251(人脑胶质瘤)细胞,研究快中子诱发U251的相关生物效应;方法:快中子照射U251细胞,吸收剂量分别为1、3、5、7 Gy,辐照后检测细胞的存活率、凋亡率、生长曲线以及周期变化等相关指标;结果:随着吸收剂量的增加,细胞存活率下降,凋亡率上升,呈良好的剂量-效应关系。照射后24 h,细胞生长曲线无明显变化;照射后48 h,细胞增殖速度明显变缓;结论:快中子为高LET射线,与相关文献报道的γ射线相比,快中子具有较高的相对生物效应。Objective： U251 cells were exposed to fast neutrons of 14 MeV energy,and dose-response relationships were derived;Methods：cell samples were exposed to fast neutrons.Radiation doses were 1 Gy,3 Gy,5 Gy and 7 Gy respectively.The cell survival,apoptotic rate and proliferation were tested after irradiation;Result： with the growth of the radiation dose,cell survival was declined,and apoptotic rate was promoted;the speed of the cell proliferation,which was observed 48 h after irradiation,was slowed down obviously;Conclusion： compared to γ ray,fast neutrons have higher relative biological effectiveness.

关 键 词：快中子 U251细胞 生物效应 剂量效应曲线 

分 类 号：R811.5[医药卫生—放射医学]