广西猪源禽Ⅰ型副粘病毒的分离与鉴定  

Isolation and identification of porcine origin avian paramyxovirus serotype 1 in Guangxi

作　　者：杨荣[1] 何奇松[1] 伍和明 冯淑萍[1] 付薇[1] 徐贤坤[1] 蒋家霞[3] 孙翔翔[3] 熊毅[1] 

机构地区：[1]广西动物疫病预防控制中心,南宁530001 [2]桂林市动物疫病预防控制中心,广西桂林541002 [3]广西大学动物科学技术学院,南宁530005

出　　处：《南方农业学报》2012年第7期1041-1045,共5页Journal of Southern Agriculture

基　　金：广西科技攻关项目(桂科攻0815009-3-7)

摘　　要：【目的】了解广西猪源禽Ⅰ型副粘病毒的流行特征、毒力强弱及基因类型,为今后开展猪源副粘病毒的相关研究提供参考,也为有效防控副粘病毒感染奠定基础。【方法】采集疑似发病猪组织病料,通过鸡胚接种传代分离病毒后,分别进行血凝(HA)与血凝抑制(HI)试验、平均死亡时间(MDT)和脑内接种致死指数(ICPI)测定,然后运用RT-PCR对分离株进行鉴定及扩增部分F基因片段,并对扩增片段进行克隆测序及比对分析。【结果】从广西疑似发病猪组织中分离获得一株猪源禽I型副粘病毒,其HA、HI效价均为26,鸡胚最小致死量为10-8/0.1mL,MDT为60h,ICPI为1.45;分离株F基因112～117位裂解位点的氨基酸组成为R-R-Q-R-R-F,与NDV标准强毒株HER33、F48E9的裂解位点一致;同源性分析结果表明,分离株与标准强毒株F48E9、中等毒力代表毒株BeaudetteC的核苷酸同源性分别为86.5%和85.7%,其推导氨基酸同源性分别为90.1%和90.8%。【结论】猪源禽Ⅰ型副粘病毒分离株为强毒株,属于基因Ⅱ型,是传统型毒株,广西地区禽I型副粘病毒宿主范围呈不断扩大趋势。[Objective]The present experiment was conducted to further understand the epidemic features, toxicity levels, and genotypes of porcine origin avian paramyxovirus serotype 1 in Guangxi in order to provide the relevant data for carrying out future researches on porcine paramyxovirus, and to provide references for the prevention and control of the disease. [Method]The virus was isolated from suspected samples of pigs using chicken embryos inoculation. The characteristics of the virus were determined with hemagglutination （HA）, hemagglutination-inhibition （HI） test, mean death time of chicken embryos （MDT）, and intracerebral phthogenicity index （ICPI）. The virus strain was identified and F gene fragments were amplified using RT-PCR and sequenced. [Result]The pig source avian paramyxovirus serotypeⅠisolate was isolated successfully from suspected samples of pigs. Its titer of HA and HI was 26 and chick embryo minimal lethal dose was 10-8/0.1 mL. The MDT and ICPI of this isolate were 60 h and 1.45, respectively. Amino acid composition was R-R-Q-R-R-F of F gene 112-117 splitting sites, which was in accordance with those of NDV standard virulent strain HER33 and F48E9. The nucleotide homologues were 86.5 and 85.7%, respectively, and the amino acid homologues were 90.1 and 90.8% after comparing the F gene of strain with standard virulent strain F48E9 and moderate virulence strain Beaudette C. [Conclusion]The isolate was virulent strain belonging to A PMV-1 gene typeⅡand traditional strains. It signified that hosts of APMV-1 were spreading and expanding constantly in Guangxi area.

关 键 词：猪源禽Ⅰ型副粘病毒 分离 鉴定 序列分析 F基因 广西 

分 类 号：S852.659.5[农业科学—基础兽医学]