银杏达莫对人腹膜间皮细胞凋亡与TGF-β活性的影响  

Effects of Ginkgo Leaf Extract and Diphyridamole on Apoptosis and TGF-β Activity of Human Peritoneal Mesothelial Cells

作　　者：李明 尹丽敏 

机构地区：[1]浙江省温岭市第一人民医院药物室,317500

出　　处：《医药导报》2012年第8期979-982,共4页Herald of Medicine

基　　金：温岭市科技局基金项目(2010WLCA0062)

摘　　要：目的探讨银杏达莫对体外培养的人腹膜间皮细胞(HPMC)β转化生长因子(TGF-β)活性的影响。方法采用细胞培养技术,以HPMC为靶细胞,以不同浓度银杏达莫处理48 h,WST-8法测定银杏达莫对HPMC增殖的影响;荧光染料Hoechst33258染色,荧光显微镜观察细胞核碎片;流式细胞仪分析细胞周期变化;酶联免疫法检测TGF-β浓度。结果 10和100μmol·L-1银杏达莫对HPMC生长有明显抑制作用,且可诱导HPMC细胞凋亡,降低TGF-β基因活性[实验前(242.20±0.13),实验后(88.78±0.14),P<0.05)]。结论银杏达莫能诱导HPMC细胞凋亡,其机制可能与TGF-β依赖性凋亡调节信号通路有关。Objective To study the effect of ginkgo leaf extract and diphyridamole on the proliferation and TGF-β activity of human peritoneal mesothelial cells（HPMCs）.Methods HPMCs were treated with ginkgo leaf extract and diphyridamole injection for 48 h and observed under a microscope by using Hoechst 33258 staining.The cell proliferation was detected with WST-8 method.Apoptosis peaks were examined by flow cytometry.TGF-β activity was detected by ELISA.Results Treatment of 10 and 100 μmol·L-1 ginkgo leaf extract and diphyridamole could inhibit the proliferation of HPMCs,induce HPMC apoptosis and down-regulate the TGF-β activity（before the treatment： 242.20±0.13,after the treatment： 88.78±0.14,P〈0.05）.Conclusion Ginkgo leaf extract and diphyridamole at 10 and 100 μmol·L^-1 induces apoptosis of HPMCs,which is dependent on TGF-β pathway in the HPMCs.

关 键 词：银杏达莫 腹膜透析 人腹膜间皮细胞 凋亡 Β转化生长因子 

分 类 号：R286[医药卫生—中药学] R965[医药卫生—中医学]