高效液相色谱法同时测定复方丹参提取液中丹参素、原儿茶醛的含量  

Simultaneous Determination of Danshensu and Protocatechuic Aldehyde in Compound Danshen Extract by HPLC

作　　者：陈赛红[1] 马晓鹂[1] 陈广斌[2] 林坚涛[3] 

机构地区：[1]广东医学院附属医院药学部,广东湛江524001 [2]深圳市龙岗区人民医院药剂科,广东深圳518172 [3]广东医学院中药研究所,广东东莞523000

出　　处：《中国医院用药评价与分析》2012年第7期627-629,共3页Evaluation and Analysis of Drug-use in Hospitals of China

摘　　要：目的:建立高效液相色谱法(HPLC)同时测定复方丹参提取液中丹参素、原儿茶醛的含量。方法:Agilent C18柱(250 mm×4.6 mm,5μm),1%醋酸溶液-甲醇采用梯度洗脱,检测波长280 nm,流速1 mL·min-1。结果:复方丹参提取液中的杂质对丹参素、原儿茶醛的测定无干扰,各组分在各自的线性范围内均呈良好的线性关系(r>0.999),平均加样回收率、精密度、重现性和稳定性均符合有关规定。结论:本方法操作简便,结果准确,可以作为控制复方丹参内在质量的方法。OBJECTIVE：To establish HPLC for simultaneous determination of Danshensu and protocatechuic aldehyde in compound Danshen extract.METHODS： The separation of samples was performed on an Agilent C18（250 mm×4.6 mm,5 μm） column with gradient elution for mobile phase which consisted of 1% acetic acid solution-methanol at a flow rate of 1 mL·min-1.The UV detective wavelength was set at 280 nm.RESULTS： Danshensu and protocatechuic aldehyde in compound Danshen extract were found to be free of interference from the foreign material.The calibration curves all were linear（r〉0.999） within their respectively range.The method recovery,reproducibility and stability of the samples were all up to the standard.CONCLUSION： The method is rapid and accurate and it can be used to control the internal quality of compound Danshen.

关 键 词：复方丹参 高效液相色谱法 丹参素 原儿茶醛 含量测定 

分 类 号：R932[医药卫生—生药学]