脂多糖干预后人皮肤成纤维细胞的胶原代谢  

Influence of Lipopolysaccharide on Collagen Metabolism of Normal Skin Fibroblasts of Human

作　　者：李凤玉[1] 王舒琦[1] 贾国洪[1] 万立[1] v 杨红明[1] 

机构地区：[1]解放军251医院肿瘤科,河北张家口075000

出　　处：《华南国防医学杂志》2012年第4期308-310,341,共4页Military Medical Journal of South China

基　　金：国家自然科学基金项目(30371467)

摘　　要：目的探讨脂多糖(lipopolysaccharide,LPS)对皮肤成纤维细胞胶原代谢的影响,以了解LPS在增生性瘢痕形成中的生物学作用。方法取正常皮肤行成纤维细胞培养后,分为1个对照组及6个实验组。实验组终浓度分别为0.005、0.010、0.050、0.100、0.500和1.000μg/ml大肠杆菌LPS(E.coli055:B5)培养,对照组为DMEM培养。用逆转录-聚合酶链反应(reverse transcription-polymerase chain reaction,RT-PCR)测定成纤维细胞Ⅰ、Ⅲ型前胶原mRNA及胶原酶mRNA的表达,并以同一个体相同代数的瘢痕组织成纤维细胞做对照。结果与对照组比较,0.005～0.500μg/ml组促进细胞胶原合成,且0.100μg/ml组作用达高峰(P<0.05),1.000μg/ml LPS抑制细胞胶原合成(P<0.05)。LPS刺激浓度在0.005μg/ml～0.100μg/ml时,促进正常皮肤成纤维细胞Ⅰ、Ⅲ型前胶原mRNA表达,抑制胶原酶mRNA表达,且呈一定的剂量依赖性;当LPS刺激浓度为0.500μg/ml,上述作用下降;而当LPS刺激浓度到达1.000μg/ml时,抑制正常皮肤成纤维细胞Ⅰ、Ⅲ型前胶原mRNA表达,促进胶原酶mRNA表达。LPS刺激浓度在0.100μg/ml时,成纤维细胞Ⅰ、Ⅲ型前胶原mRNA和胶原酶mRNA表达与同一个体增生性瘢痕组织成纤维细胞近似。结论 LPS对人皮肤成纤维细胞Ⅰ、Ⅲ型前胶原mRNA和胶原酶mRNA表达的直接调节,可能是其参与增生性瘢痕形成的重要机制。Objective To observe the influence of lipopolysaccharide（LPS） on collagen metabolism of normal human skin fibroblasts and its biological role in the formation of hypertrophic scar.Methods Fibroblasts were isolated and cultured in vitro,and then exposed to different doses of LPS（0.005,0.010,0.050,0.100,0.500 and 1.000 μg/ml） from E.coli055：B5 respectively.The expression of procollagen type Ⅰ,Ⅲ and collagenase mRNAs was tested by reverse transcription-polymerase chain reaction（RT-PCR）.The fibroblasts from hypertrophic scar tissue obtained from the same patients in the same culture passage were used as control.Results Compared with the control group,The expression of procollagen type Ⅰ,Ⅲ mRNAs of normal skin fibroblasts was increased,while the expression of collagenase mRNAs of normal skin fibroblasts was depressed when challenged with LPS at the concentration of 0.005 μg/ml,and the influence was concentration dependent.When the LPS concentration of LPS was 0.500 μg/ml,the influence of LPS on the expression of procollagen type Ⅰ,Ⅲ and collagenase mRNAs of normal skin fibroblasts began to decrease.When the LPS concentration of LPS reached 1.000 μg/ml,the expression of procollagen type Ⅰ,Ⅲ mRNAs was inhibited and the expression of collagenase mRNAs began to increase.When the LPS concentration was 0.100 μg/ml,the expression of procollagen type Ⅰ,Ⅲ and collagenase mRNAs of normal human skin fibroblasts was similar to that of the hypertrophic scar tissue fibroblasts.Conclusion LPS may be an important factor controlling collagen metabolism of normal skin fibroblasts and hypertrophic scar formation.

关 键 词：脂多糖 成纤维细胞 胶原代谢 

分 类 号：R318[医药卫生—生物医学工程]