聚(3,4-乙烯基二氧噻吩)/碳纳米管复合膜修饰电极的电化学制备及其用于8-羟基-2'-脱氧鸟嘌呤核苷的检测  

Electrochemical Preparation of Poly(3,4-Ethylenedioxythiophene)/Carbon Nanotubes Composite Film Modified Electrode and Its Application to Determination of 8-Hydroxy-2′-Deoxyguanosine

作　　者：潘吉超[1] 姚飞[1] 任腾飞[1] 贾丽萍[1] 贾文丽[1] 崔慧[1] 王怀生[1] 

机构地区：[1]聊城大学化学化工学院,山东聊城252059

出　　处：《南京师大学报（自然科学版）》2012年第2期66-71,共6页Journal of Nanjing Normal University(Natural Science Edition)

基　　金：国家自然科学基金(21075059);山东省"泰山学者"建设工程专项经费资助

摘　　要：通过恒电流电解一步法在玻碳电极表面制备了聚(3,4-乙烯基二氧噻吩)/碳纳米管复合膜,实验结果表明,该复合膜修饰电极综合了聚(3,4-乙烯基二氧噻吩)(PEDOT)和碳纳米管(CNTs)两者的优点,对8-羟基-2'-脱氧鸟嘌呤核苷(8-OH-dG)的氧化具有明显的增强作用,较好地抑制尿酸的干扰,而且具有很好的重现性和稳定性.在0.1 mol/L pH 9.0的磷酸盐缓冲溶液(PBS)中,8-OH-dG的氧化峰电流与其浓度在0.014～14.0μmol/L和14.0～56.0μmol/L两个范围内成良好的线性关系,检出限可达35 nmol/L(S/N=3).The poly（3, d-ethylenedioxythiophene）/carbon nanotubes （CNTs） composite film was prepared on the glassy carbon electrode surface by one-step galvanostatic deposition and used for the study of the electrochemical behavior of 8- hydroxy-2＇-deoxyguanosine （8-OH-dG）. The experimental results showed that the hybrid film can combine the advanta- ges of conductivity of PEDOT and the unique electronic performance of CNTs and effectively enhance the adsorption and electroc, hemical oxidation of 8-OH-dG. Moreover, this proposed hybrid fihn modified electrode also showed excellent re- producibility and stability in the experiment. In 0. 1 mol/L pH 9. 0 phosphate buffer solution, the oxidative peak currents of 8-OH-dG had two good linear relationships with its concentration in 0. 014 - 14 μmol/L and 14 -56 μmol/L, with the detection limit of 35 nmol/L （ S/N = 3 ）.

关 键 词：8-羟基-2’-脱氧鸟嘌呤核苷 聚(3 4-乙烯基二氧噻吩) 碳纳米管 导电聚合物 尿酸 

分 类 号：O657.1[理学—分析化学]