中华绒螯蟹螺原体对中华绒螯蟹和RAW264.7细胞免疫反应研究  

The Immune Responses in Chinese Mitten Crab Eriocheir Sinensis and RAW264.7 Cell Line Challenged With Spiroplasma Eriocheiris

作　　者：孟庆国[1] 黄艳青[2] 靳明建 顾伟[1] 王文[1] 

机构地区：[1]南京师范大学生命科学学院,江苏省生物多样性与生物技术重点实验室,江苏省水生甲壳动物病害重点实验室,江苏南京210046 [2]中国水产科学研究院东海水产研究所,农业部海洋与河口渔业资源及生态重点开放实验室,上海200090 [3]江苏省如东县渔业技术推广站,江苏南通226400

出　　处：《南京师大学报（自然科学版）》2012年第2期77-82,88,共7页Journal of Nanjing Normal University(Natural Science Edition)

基　　金：国家自然科学基金(31170120);江苏省自然科学基金(2011104SBZ0122);江苏省普通高校自然科学基金(2011104TSJ0154);江苏省水产三项工程项目(PJ2011-65)

摘　　要：中华绒螯蟹螺原体是一种引起中华绒螯蟹颤抖病的新型病原微生物,对中华绒螯蟹养殖业造成了很大的损失.虽然很多无脊椎动物免疫反应的研究已经报道,但是螺原体引起宿主免疫反应的研究目前还未见报道.中华绒螯蟹螺原体既可以感染中华绒螯蟹,也可以感染乳鼠.本文在动物个体水平上研究了中华绒螯蟹螺原体与中华绒螯蟹的免疫关系,中华绒螯蟹经螺原体刺激后,其体内抗脂多糖因子、酚氧化酶原、酚氧化酶原激活因子、消极素轻链等基因的mRNA的表达水平显著上升,而抗氧化蛋白Prx 6的表达量显著下降.本文还在细胞水平研究了中华绒螯蟹螺原体与小鼠巨噬细胞之间的免疫关系,中华绒螯蟹螺原体刺激后,细胞中CD40、IL-1β、IL-10基因的mRNA表达量显著下降,而TGF-β1和TNF-α等基因的表达量上升.本实验结果说明螺原体可以引起寄主很广泛的免疫反应.Spiroplasma eriocheiris is a new kind of pathogen in aquacuhure and causes mass mortality of Chinese mitten crab Eriocheir sinensis, but little is known about the immune responses in E. sinensis challenged with this agent. Al- though immune responses in invertebrates have been widely studied, spiroplasma-induced innate immune responses have not been reported. The mRNA expressions of anti-lipopolysaccharide factor （ALF）, prophenoloxidase （proPO）, proph- enoloxidase-activation factor （PPAF）, pacifastin light chain （PLC） and peroxiredoxin 6 （Prx6） immune-related genes were examined in order to estimate the effects of S. eriocheiris on the innate immunity of E. sinensis. All of the mRNA expressions of above genes except for Prx6 were significantly up-regulated after S. eriocheiris challenge. But the mRNA expression of Prx6 was significantly down-regulated after the same treatment. S. eriocheiris not only cause tremor disease of E. sinensis, but also can cause suckling mouse cataract just like Spiroplasma mirum. So we chose RAW264. 7 cell line, a murine macrophage-like cell, to study the relationship between the S. eriocheiris and the host in cell level. The expression levels of CIM0 ,IL-1β ,IL-10 in RAW264. 7 cells were up-regulated after challenge with S. eriocheiris, while the expression levels of TGF-β1 and TNF-α were down-regulated. The resuhs suggested that broad-spectrum immune responses could be induced by S. eriocheir in both E. sinensis and RAW264. 7cell.

关 键 词：中华绒螯蟹 RAW264.7细胞 螺原体 免疫反应 

分 类 号：Q936[生物学—微生物学]