气相色谱法测定磺胺多辛中的冰醋酸和N,N-二甲基甲酰胺残留  

Determination of Residual Acetic Acid and N,N-Dimethylformamide in Sulfadoxine by Capillary Gas Chromatography

作　　者：井玥[1] 王刚[1] 张爱莲[1] 盛晓玲[1] 龚祝南[1] 

机构地区：[1]南京师范大学生命科学学院,江苏南京210046

出　　处：《南京师大学报（自然科学版）》2012年第2期93-96,共4页Journal of Nanjing Normal University(Natural Science Edition)

摘　　要：目的:建立磺胺多辛原料药中冰醋酸、N,N-二甲基甲酰胺(DMF)有机溶剂残留量的气相色谱测定方法.方法:样品用甲醇溶解后,在DB-WAX毛细管柱(30 m×0.32 mm×0.5μm)上实现分离(FID检测器).结果:2种溶剂残留均达到良好分离,冰醋酸和DMF线性范围分别为Y=3.565 7X+4.078 4(r=0.999 3)、Y=7.248 9X-7.488 9(r=0.999 6),精密度RSD分别为1.8%、3.3%,回收率分别为99.7%、100.1%,检测限分别为35.46ng/mL、66.5 ng/mL.结论:本方法准确、可靠、灵敏度高,适用于磺胺多辛中冰醋酸与N,N-二甲基甲酰胺残留量的检测.Objective： To establish a method for the determination of residual organic solvent, acetic acid and NN-Dime- thylformamide（DMF） in sulfadoxine. Method： Capillary gas chromatographic method was introduced for the determina- tion, residual organic solvent was extracted from sulfadoxine with methanol and separated in DB-WAX capillary column with FID detector. Result ： the linear relation of acetic acid and NN-Dimethylformamide was Y = 3. 565 7X ＋ 4. 078 4 （ r =0.9993） and Y=7.2489X-7.4889 （r =0.9996）, with accuracy RSD （1.8% and 3.3%）, recovery rate （99.7% and 100.1% ） , LOD （35.46 ng,/mL and 66.5 ng/mL） respectively. Conclusion： The method was accurate, reliable and high sensitivity for determination of residual organic solvent in sulfadoxine.

关 键 词：磺胺多辛 冰醋酸 N N-二甲基甲酰胺 毛细管气相色谱 残留 

分 类 号：R927.11[医药卫生—药学]