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机构地区:[1]贵州大学昆虫研究所/贵州山地农业病虫害重点实验室,贵阳550025 [2]遵义医学院,贵州遵义563003
出 处:《湖北农业科学》2012年第15期3355-3357,共3页Hubei Agricultural Sciences
基 金:国家自然科学基金项目(30100015;30560020;31160163);贵州省优秀青年科技人才基金项目(黔科合人字[2005]0520);贵州省优秀科技教育人才省长资金(黔省专合字[2005]357);贵州省国际科技合作项目(黔科合外G字[2010]7005)
摘 要:通过PCR扩增获得了飞虱科3属5种昆虫的16 S rDNA序列,对DNA序列进行测定,分析了其序列组成及变异。采用Mega 4.0软件,利用邻接法(NJ)和最大简约法(MP)进行5种飞虱的系统发育分析,结果显示构建的MP和NJ分子系统树拓扑结构一致,3个簇角飞虱属物种中华簇角飞虱(Belocera. sinensis)、褐额簇角飞虱(B.fuscifrons)和爬竹簇角飞虱(B.ampelocalamus)单独聚为一支,与偏角飞虱属海南偏角飞虱(Neobelocera hainanensis)的亲缘关系较近,与短头飞虱属显脊短头飞虱(Epeurysa distincta)的亲缘关系较远,分子生物学分析结果与形态学研究结果一致。The 16 S rDNA sequences of 5 plant hoppers belonging to 3 genus in the family of Delphacidae were obtained by PCR and then sequenced. The sequence composition and mutation were analyzed. Molecular phylogenetic trees were construct- ed by maximum parsimony method (MP) and neighbor-joining method (NJ) using Mega 4.0 software. The result showed that the topologies of MP tree and NJ tree constructed were the same. Belocera Sinensis, B. fusc/frons and B. ampeloca!amus were clustered into one group, which had closer relationship to Neobelocera hainanensis while further distance from Epeurysa dis- tinctca These results were identical with those of morphology research.
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