大花蕙兰原球茎的诱导、增殖及植株再生研究  

Study on Protocorm Induction,Proliferation and Plantlet Formation of Cymbidium hybridum

作　　者：季华[1] 吴伟剑 吴华[1] 陈龙清[1] 

机构地区：[1]华中农业大学园艺林学学院/园艺植物生物学教育部重点实验室,武汉430070 [2]武汉市黄陂区武湖农场,武汉430345

出　　处：《湖北农业科学》2012年第15期3369-3371,3405,共4页Hubei Agricultural Sciences

基　　金：中央高校基本科研业务费专项资金(2010QC028);华中农业大学硕士研究生创新研究项目(3003-457);湖北省科技攻关重大项目(2006AA205A04)

摘　　要：从大花蕙兰(Cymbidium hybridum)试管苗取材,诱导原球茎,建立植株再生体系,分析了不同激素及其浓度对原球茎诱导、增殖和植株生根的影响。结果表明,不定芽比叶片更容易诱导出原球茎;生长素NAA对原球茎的诱导效果好于2,4-D,最适合原球茎诱导的激素组合为1.0 mg/L BA+1.0 mg/L NAA;原球茎增殖培养基中添加1.0 mg/L BA+0.5 mg/L NAA增殖效果较好,增殖系数可达7.90;最适生根培养基为1/2 MS+0.5 mg/L NAA+30 g/L蔗糖。Explants were drawing from Cymbidium hybridum plantlet for protocorm induction and plantlet regeneration. Effects of the kind and concentration of plant growth regulators on protocorms induction, proliferation and root formation were ana- lyzed. The results showed that introducing protocorm from adventitious bud was more easily than from leaf primordial. NAA was more effective for protocorm induction than 2,4-D. The optimum hormone combination for protocorm induction was 1.0 mg/L BA＋I.0 mg/L NAA. The multiplication coefficient of protocorm was the highest （7.90） in medium adding 1.0 mg/L BA＋ 0.5 mg/L NAA. The optimal medium for root formation was 1/2 MS＋0.5 mg/L NAA＋ 30 g/L sucrose.

关 键 词：大花蕙兰(Cymbidium hybridum) 原球茎 组织培养 植株再生 

分 类 号：S682.31[农业科学—观赏园艺] S603.6[农业科学—园艺学]