草鱼肠道芽孢杆菌的鉴定及产酶能力分析  

Study on bacillus from grass carp(Ctenopharyngodon idellus) intestine and its zymogenicities

作　　者：贺国龙[1] 刘立鹤[1] 张恒[1] 

机构地区：[1]武汉工业学院,湖北省动物营养与饲料科学重点实验室,武汉430023

出　　处：《淡水渔业》2012年第4期3-8,共6页Freshwater Fisheries

基　　金：武汉工业学院研究生科研创新资金(2010cx020)

摘　　要：从100 g左右的健康草鱼(Ctenopharyngodon idellus)肠道中分离出3株疑似芽孢杆菌(Bacillus)菌株(G1、G2、G3),并通过对其进行生理生化特征、16S rDNA全序列和产酶能力的分析,筛选出1株高产酶能力的益生菌株。结果显示:G1、G2和G3菌株与枯草芽孢杆菌在16S rDNA序列相似性≥99%的水平上聚为同一分支,结合形态观察和生理生化特征,最终鉴定G1、G2和G3菌株均为枯草芽孢杆菌(Bacillus subtilis);以水解圈直径/菌落直径比值评定了G1、G2和G3菌株的产纤维素酶、淀粉酶和蛋白酶的能力,其中G1菌株产纤维素酶、淀粉酶和蛋白酶能力(比值分别为2.9、2.2和3.3)均高于G2、G3菌株,具有作为益生菌的潜力。Three suspected Bacillus strains （G1, G2, G3 ） were isolated from intestine of healthy grass carp （100g）, and one high ectoenzyme-producing bacillus strain was screened by the way of biochemistry characteristic, 16S rDNA se- quences and ectoenzyme-producing analysis. The results showed that G1, G2, G3 and Bacillus subtilis were clustered at similarity levels （ = 99% ） of 16S rDNA sequences. The identification showed that all of G1, G2, G3 were Bacillus subti- l/s by the way of morphology, biochemistry characteristic and 16S rDNA sequences homology analysis. The ability of the G1, G2 and G3 strains producing cellulase, amylase and protease were assessed by D/d （ D ： diameler of hydrolysis trans- parent circle; d： colony diameter）, in which G1 had higher zymogenicities of cellulase, protease, amylase （2.9, 2.2 and 3.3, respectively） than G2, G3, with potential as probiotics.

关 键 词：草鱼(Ctenopharyngodon idellus) 芽孢杆菌 产酶能力 

分 类 号：S917.1[农业科学—水产科学]