茵陈蒿汤HPLC指纹图谱初步研究  

Preliminary Studies on HPLC Fingerprint of Yinchenhao Decoction

作　　者：杨爱华[1] 窦志华[2] 罗琳[3] 葛建华 孟萍[2] 

机构地区：[1]南京中医药大学,南京210046 [2]南通大学附属南通第三医院,南通226006 [3]南通大学,南通226001 [4]南通市药品检验所,南通226006

出　　处：《药学与临床研究》2012年第4期301-303,共3页Pharmaceutical and Clinical Research

基　　金：江苏省高校自然科学基础研究项目(08KJB360009);江苏省中医药局中医药科学技术研究专项课题(HZ07071)

摘　　要：目的:优选茵陈蒿汤HPLC指纹图谱色谱条件。方法:采用welch-materials XB-C18(250 mm×4.6 mm,5μm)色谱柱,乙腈(A)-0.1%磷酸(B)梯度洗脱,流速1.0 mL·min-1,柱温30℃,检测波长254 nm,以标准品对照法初步鉴定茵陈蒿汤主要有效成分。结果和结论:该色谱条件下,茵陈蒿汤指纹图谱中8个成分得到鉴定,其中没食子酸、芦荟大黄素、大黄酸、大黄素、大黄酚及大黄素甲醚来源于大黄,绿原酸和栀子苷来源于茵陈、栀子。Objective： To screen the HPLC chromatographic condition for establishing Yinchenhao decoction （YCHD） fingerprint. Methods： The method was established on a Welch-materials XB-C18 column （250 mm×4.6 mm, 5 μm） with mobile phase in gradient elution composed of acetonitrile （A）-0.1% phosphoric acid （B） flowed at 1 mL.min-1. The column temperature was 35℃ and the detection wavelength was 254 nm. The main active ingredients of YCHD were identified preliminarily by comparing with standard controls. Results and Conclusion： In this chromatographic condition, eight components in YCHD have been identified as galic acid, chlorogenic acid, geniposide, aloe-emodin, rhein, emodin, chrysophanol and physcion.

关 键 词：茵陈蒿汤 高效液相色谱法 指纹图谱 

分 类 号：R927[医药卫生—药学]