松花粉对D-半乳糖衰老模型小鼠学习记忆功能的影响及机制研究  

Effect of Pine Pollen on Learning and Memory Function in D-galactose-induced Aged Mice as well as Its Related Mechanisms

作　　者：毛根祥[1] 曹永葆[1] 何志华 王雅珍[1] 胡细连[1] 吕远栋[1] 徐伟红[1] 王国付[1] 

机构地区：[1]浙江医院浙江省老年医学重点实验室,310013 [2]浙江省林业技术推广总站,310020

出　　处：《心脑血管病防治》2012年第4期290-293,共4页CARDIO-CEREBROVASCULAR DISEASE PREVENTION AND TREATMENT

基　　金：卫生部科学研究基金(编号:WKJ2011-2-014);浙江医院医药卫生科研基金(编号:2011YB005);浙江省医学高等专科学校科研基金(编号:2009XZB09)

摘　　要：目的探讨松花粉对脑衰老的作用及其相关机制。方法以100mg/kg D-半乳糖皮下注射建立小鼠衰老模型,灌胃给予模型小鼠高、中、低三个剂量(500mg/kg、1000mg/kg、1500mg/kg)的松花粉,并以非酶糖基化抑制剂盐酸氨基胍(AG)为阳性对照,连续给药8周后,采用跳台法测定小鼠的学习记忆能力。处死小鼠获取血清及脑组织,检测晚期糖基化终末产物(AGE)水平,超氧化物歧化酶(SOD)活力、MDA水平以及炎症因子IL-6和TNF-α水平等衰老相关指标。结果跳台法检测模型组较正常组潜伏期显著缩短,5min内错误次数显著增加(P<0.05),而松花粉处理后能显著逆转这一变化(P<0.05),高剂量组效果最佳;同时,松花粉处理组能显著抑制模型组血清及脑组织中AGE水平及IL-6、TNF-α水平的升高(P<0.05),提高血清及脑组织SOD活力(P<0.05)并下调MDA水平(P<0.01)。结论松花粉能显著改善衰老模型小鼠的学习记忆功能,并上调抗氧化活性和降低衰老相关的炎症介质水平,抑制体内非酶糖基化可能是其发挥延缓衰老作用的机制之一。Objective To investigate the effects of pine pollen against cerebral aging as well as its related mechanisms in an accelerated mouse aging model induced by D-galactose. Methods A group of 4-month-old C57BL/6J mice were treated daily with D-galactose, D-galactose combined with various dosages of pine pollen （500mg/kg, 1000mg/kg, 1500mg/kg）, D-galactose combined with AGE formation inhibitor aminoguanidine（AG）, and control buffer for 8 weeks. At the end of the treatment, the learning and memory of mice were detected by step-down test. Serum and cerebral AGE concentration, SOD activity, MDA content as well as IL-6 and TNF- levels were measured. Results D-galactose induced mouse aging model was developed as described before. Pine pollen reversed D- galactose induced aging effects in neural activity and inflanmaatory cytokine levels, as evidenced by improved memory latency time and reduced error rale in step-down test （ P 〈 0.05）. and decreased concentrations of IL-6 and TNF-a both in serum and brain in aged model mice （ P 〈 0.05）. Pine pollen blocked D-galactose induced increase of AGE levels in senml and brain. Further more, the de- clined anti-oxidant activity in tbe aged model mice was also partly reversed upon pine pollen treatment （ P 〈 0.05 ）. Conclusions Pine pellen inhibits AGE fomlation in vivo, which at least partially contributes to its beneficial effect on cerebral aging in a D-galactose in- duced aging model.

关 键 词：松花粉 D-半乳糖 衰老 小鼠 晚期糖基化终末产物 

分 类 号：R285.5[医药卫生—中药学]