犀黄丸对大肠癌LoVo细胞基质金属蛋白酶2,9蛋白表达的影响  

Effect of Xihuang pills on protein expressions of MMP-2 and MMP-9 in human colorectal carcinoma LoVo cell

作　　者：孙莉娜[1] 孟静岩[1] 王威[1] 应森林[1] 李丹[1] 马佐英[1] 贾彦焘[2] 

机构地区：[1]天津中医药大学,天津300193 [2]天津中医药大学第一附属医院,天津300193

出　　处：《天津中医药》2012年第4期378-380,共3页Tianjin Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(3087227)

摘　　要：[目的]研究犀黄丸含药血清对大肠癌LoVo细胞中基质金属蛋白酶-2,9(MMP-2,MMP-9)表达的影响及意义。[方法]应用酶联免疫吸附试验(ELISA)方法,检测犀黄丸含药血清作用下LoVo细胞培养上清液中MMP-2、MMP-9的蛋白表达水平。[结果]在犀黄丸含药血清的干预下,LoVo细胞中MMP-2、MMP-9的蛋白分泌水平与空白对照组比较明显下调,差异具有统计学意义(P<0.05)。[结论]犀黄丸可能通过抑制MMP-2和MMP-9的蛋白表达,达到抑制大肠癌转移的作用。[Objective] To investigate the effect of Xihuang pills（medicine-contained serum） on the protein expressions of matrix metallo- proteinase-2（MMP-2） and matrix metailoproteinase-9（MMP-9） in human colorectal carcinoma LoVo cell. [Methods] The enzyme-linked immunoassay （ELISA） was used to determine the expression of MMP-2 and MMP-9 in the culture solution of LoVo cell under the action of Xihuang pills. [Results] The expression of MMP-2 and MMP-9 in medication groups was significantly lower than those of control group （P〈0.05）. [Conclusion] Xihuang pill has a potent effect of reducing the expressions of MMP-2 and MMP-9. This mechanism may play an important role in the suppression of colorectal carcinoma metastasis.

关 键 词：LOVO细胞 犀黄丸 含药血清 基质金属蛋白酶-2  9 

分 类 号：R285.5[医药卫生—中药学]