益脑康对动脉粥样硬化性急性脑缺血大鼠血清NO、VEGF及PDGF-BB含量的影响  

Effects of Yinaokang Capsule on NO,VEGF,PDGF-BB content in serum of rats with atherosclerotic acute cerebral ischemia

作　　者：朱磊[1] 张彦红[2] 时光[3] 陈杰[4] 罗恩丽 黄燕 

机构地区：[1]广州中医药大学,广州510405 [2]广州市第一人民医院,广州510180 [3]广州中医药大学第一附属医院,广州510405 [4]佛山市中医院,佛山528000 [5]广州中医药大学第二附属医院,广州510120

出　　处：《中华中医药杂志》2012年第8期2048-2051,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家科技部"十五"攻关课题(No.2004BA721A02)~~

摘　　要：目的:观察中药益脑康胶囊对动脉粥样硬化(AS)基础上急性脑缺血(ACI)SD大鼠血清一氧化氮(NO)、血管内皮生长因子(VEGF)、血小板衍化生长因子-BB(PDGF-BB)含量的影响,以探讨益脑康胶囊防治缺血性中风的可能机制。方法:将体质量(180±20)g SD雄性大鼠随机分为A、B、C、D、E、F(即正常对照组、益脑康预防组、AS模型组、AS+ACI模型组、益脑康治疗组和立普妥治疗组)6组,采用腹腔注射VD3+高脂喂养复制AS模型,内皮素-1在大脑中动脉附近注射法复制ACI模型。B组在造模同时给予益脑康灌胃治疗,其余各组大鼠在模型成功后分别予0.9%氯化钠溶液、益脑康和立普妥灌胃治疗1周,在治疗前后分别测定血清NO、VEGF、PDGF-BB的含量。结果:B组、F组大鼠血清NO含量较D组明显增高(P<0.01,P<0.05)。B组、E组、F组与D组比较,血清VEGF、PDGF-BB水平均明显下降(P<0.01,P<0.05)。结论:益脑康胶囊可通过提高血清NO含量,降低VEGF、PDGF-BB含量发挥防治动脉粥样硬化性急性缺血中风的作用。Objective：By observing the effects of Yinaokang Capsule on NO,VEGF and PDGF-BB content in serum of atherosclerotic acute cerebral ischemic SD rats,the possible mechanism of the capsule for preventing ischemic stroke is investigated.Methods：SD male rats weighted（180±20）g were randomly divided into 6 groups：A,B,C,D,E,F（Respectively for the normal control group,the Yinaokang Capsule prevention group,the AS model group,the AS ＋ ACI model group,the Yinaokang Capsule treatment group and the Lipitor treatment group）.AS model is replicated by intraperitoneal injection of VD3 ＋ high fat diet,and ACI model is replicated by the injection of ET-1 near the MCA.Rats of group B were treated with Yinaokang gastric perfusion while the modeling,the other rats were given saline,Yinaokang and Lipitor by gavage 1 week after successful modeling.The levels of NO,VEGF,and PDGF-BB in serum were measured before and after treatment.Results：NO content in serum of group B and group F was significantly higher than that of group D（P〈0.01,P〈0.05）.Compared with group D,VEGF and PDGF-BB levels in the serum of Group B,E,F were significantly decreased（P〈0.01,P〈0.05）.Conclusion：By increasing serum NO content and decreasing the content of VEGF,PDGF-BB,Yinaokang Capsule plays the role of prevention and treatment in rats of atherosclerotic acute ischemic stroke.

关 键 词：缺血性中风 益脑康胶囊 ACI动物模型 一氧化氮 血管内皮生长因子 血小板衍化生长因子 

分 类 号：R285.5[医药卫生—中药学]