回药爱康方对Lewis肺癌抑制作用及突变型p53蛋白表达的影响  

Effects of Hui medicine Aikang Fang for lewis lung carcinoma inhibition and mutant p53 expression

作　　者：马科[1] 马治国[2] 张丽娜[3] 周丽萍[3] 边静[1] 周慧[1] 

机构地区：[1]宁夏医科大学中医学院,银川750004 [2]宁夏医科大学附属银川市中医医院,银川750004 [3]宁夏医科大学总医院,银川750004

出　　处：《中华中医药杂志》2012年第8期2158-2160,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81160462);宁夏回族自治区科技攻关课题[No.(2008)80]~~

摘　　要：目的:观察回药爱康方对小鼠肿瘤生长的影响及对突变型p53蛋白(p53mt)表达的影响。方法:将55只C57小鼠常规接种Lewis肺癌细胞,24h后随机分为荷瘤模型组、化疗组、回药低剂量组、回药低剂量加化疗组、回药高剂量加化疗组。治疗后观察各组小鼠的脾脏、胸腺、瘤体质量,免疫组化法检测各组p53mt的表达差异。结果:①除化疗组外,其余各组小鼠体质量与荷瘤模型组比较差异均有统计学意义(P<0.05);②回药高剂量加化疗组抑瘤率为71.00%,与荷瘤模型组比较,各组瘤质量差异有统计学意义(P<0.05);③回药高剂量加化疗组p53mt表达阳性率为36.36%,与模型组比较有统计学意义(P<0.01);④与化疗组比较,各组小鼠脾脏指数及胸腺指数差异均有统计学意义(P<0.01)。结论:回药协同化疗抑瘤效果及下调突变型p53的表达效果优于单纯化疗,且回药爱康方可以降低化疗后的免疫抑制,这可能是回族医药治疗肿瘤的新靶点。Objective：To observe the Hui medicine Aikang Fang＇s（HAF） effects on tumor growth in mice and changes of mutant p53（mtp53） expression.Method：55 normal C57 mice were inoculated with Lewis lung cancer cells.After 24h,the mice were randomly divided into model group,chemotherapy group,the low-dose group,the low-dose with chemotherapy and high-dose with chemotherapy group.After treatment,the spleen,thymus and tumor mass of all rats were tested.Expressions of mtp53 in all groups had a significantly different using immunohistochemical method.Result：① In addition to chemotherapy group,the difference between the rest groups and model group was significant in body weight（P〈0.05）;②Inhibition rate of high-dose group was 71%.Compared with tumor-bearing model group,the difference of tumor weight in all groups were significant（P〈0.05）;③Mtp53 expression rate of high-dose with chemotherapy group was 36.36%.Compared with tumor-bearing model group,HAF high-dose with chemotherapy group was significantly different（P〈0.01）;④Compared with the chemotherapy group,spleen index and thymus index differences of the rest group mice were statistically significant（P〈0.01）.Conclusion：Inhibition of HAF with chemotherapy and effects of reducing the expression of mtp53 were better than chemotherapy alone,and HAF could reduce immune suppression after chemotherapy,it may be a new target of treatment cancer.

关 键 词：回药爱康方 LEWIS肺癌 突变型P53蛋白 实验研究 

分 类 号：R29[医药卫生—民族医学] R734.2[医药卫生—中医学]