猕猴桃根多糖抗肿瘤注射剂小鼠体内抑瘤机制  

Mechanism study of kiwifruit root polysaccharide anti-tumor injection on tumor in vivo

作　　者：张光霁[1] 韩江余[2] 

机构地区：[1]浙江中医药大学,杭州310053 [2]湖州师范学院,湖州313000

出　　处：《中华中医药杂志》2012年第8期2177-2179,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：浙江省中医药科研基金项目(No.2005C001)~~

摘　　要：目的:探寻猕猴桃根多糖抗肿瘤注射剂抑瘤作用机制。方法:采用配伍组实验设计研究方法,对采用不同剂量的用药组和对照组小鼠抑瘤率及增殖细胞核抗原(PCNA)表达进行统计分析,同时应用电镜对各组肿瘤细胞微结构进行观察。结果:猕猴桃根多糖抗肿瘤注射剂对H22肝癌细胞株呈现良好的抑制效果,中剂量组抑瘤率为68.5%,相应PCNA表达率51.20%,效果最佳。电镜显示猕猴桃根多糖抗肿瘤注射剂各实验组肿瘤细胞均出现凋亡现象,肿瘤细胞线粒体肿胀,并出现细胞胞浆内存在空泡等细胞中毒现象。结论:猕猴桃根多糖抗肿瘤注射剂对肝癌具有一定抑制作用,其机制可能是通过抑制PCNA来减缓肿瘤细胞增殖,同时对肿瘤细胞线粒体产生毒性作用。Objective：Study the Mechanism of kiwifruit root polysaccharide anti-tumor injection on tumor.Methods：Using compatibility group experimental design research methods,statistical analysis different doses of the drug group and control group mice＇s inhibitory rate and PCNA expression,and observation the cell microstructure with electron microscope.Results：kiwifruit root polysaccharide anti-tumor injection obvious inhibition on H22 tumor cell.Inhibition rate is 68.5% in the best dose group with the lowest PCNA expression of 51.20%.There is cell apoptosis in every treatment group with kiwifruit root polysaccharide anti-tumor injection,and the mitochondrial swelling in the tumor cell.At the same time there are void in cell cytoplasm.Conclusion：kiwifruit root polysaccharide anti-tumor injection inhibition the liver cancer.The mechanism maybe is inhibition PCNA to slowing down the tumor cell proliferation,while have toxic effect of mitochondrial.

关 键 词：猕猴桃根多糖 增殖细胞核抗原 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]