梭梭ARF1基因的克隆及序列分析  

Cloning and Sequence Analysis of ADP-ribosylation Factors Gene from Haloxylon ammodendron

作　　者：周晓燕[1,2] 石磊[1] 甘晓燕[1] 陈虞超[1] 宋玉霞[1] 

机构地区：[1]宁夏农林科学院,银川750002 [2]宁夏大学生命科学学院,银川750021

出　　处：《西北农业学报》2012年第7期67-71,共5页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：宁夏回族自治区科技攻关项目(KGZ-16-07-02)

摘　　要：二磷酸腺苷-核糖基化作用因子(ADP-ribosylation factors,ARFs)属小G蛋白超级家族中的Arf亚族,是真核细胞囊泡运输通道的关键组成成分,参与细胞运输和信号传导。本试验采用RT-PCR、RACE等方法从超旱生、耐盐植物梭梭(Haloxylon ammodendron)中扩增出ADP-ribosylation factor(ARF1)基因的cD-NA序列(命名为HaARF1),其开放阅读框为546bp,推测氨基酸序列全长为181个氨基酸残基,具有典型的小GTP结合蛋白结构域。其氨基酸序列与GenBank中已发表同源对比相似度达99%以上,表明ARF1基因在不同物种间高度保守。ADP-ribosylation factor（ADP-ribosylation factors,ARFs） belongs to a small G protein super-family of Arf subfamily,which is a key component of vesicle transport channel components involved in cellular transport and signal transduction in eukaryotic cells.Through RT-PCR（reverse transcription polymerase chain reaction） and RACE（rapid amplification of cDNA ends）,a 546 bp fragment containing entire ARF1 gene coding region of 181 amino acid（aa） has been obtained（named as HaARF1） from the super-xerophytic,salt-tolerant plants Haloxylon（Haloxylon ammodendron）,the deduced amino acid sequences contain a typical small GTP-binding protein domain.Encoded protein by HaARF1 and ARF1 protein from GenBank has been published homologous to other species up to 99% similarity comparison,indicating that ARF1 genes are highly conserved among different species.

关 键 词：梭梭 ADP-核糖基化作用因子 小G蛋白 序列分析 

分 类 号：S793.9[农业科学—林木遗传育种]