青蒿琥酯对Tca8113细胞的抑制作用及对RECK蛋白表达的影响  

Growth inhibition of Artesunate on Tca8113 cell and its influence on RECK expression

作　　者：周铁军[1] 向丽[2] 李健[1] 贾春霞[1] 王云[1] 龚莉[1] 

机构地区：[1]泸州医学院病理学教研室,四川泸州646000 [2]泸州医学院病原生物学教研室,四川泸州646000

出　　处：《重庆医学》2012年第23期2352-2354,2357,共4页Chongqing medicine

基　　金：四川省教育厅重点项目基金资助项目(08ZA151)

摘　　要：目的探讨青蒿琥酯(ART)对Tca8113细胞的抑制作用及对RECK蛋白表达水平的影响。方法将不同浓度的ART刺激体外培养的Tca8113细胞,MTT法检测细胞增殖情况;流式细胞术检测细胞凋亡及细胞周期情况;划痕实验检测ART对细胞迁移能力的影响;免疫细胞化学检测细胞RECK蛋白表达水平。结果不同浓度ART作用Tca8113细胞后可抑制细胞增殖,并能有效诱导细胞凋亡,且均呈现时间、剂量依赖性;ART能将Tca8113细胞阻滞在G0/G1期;ART可降低Tca8113细胞的迁移能力;高浓度ART可上调RECK蛋白的表达水平。结论 ART在体外对Tca8113细胞具有抑制作用;其可能通过上调RECK蛋白表达水平抑制肿瘤的侵袭转移。Objective To study the growth inhibition effect of artesunate on Tca8113 cells and its influence on RECK expression in vitro. Methods Different concentration ART stimulated Tca8113 cells,tested cells proliferation by MTT assay,tested cells apop tosis and cell cycle by flow cytometry, wound healing test was used to measure the cells migration ability, RECK expression was de- tected by immunocytochemical methods. Results ART not only inhibited the proliferation but also induced apoptosis of Tca8113 cells,the two were all time-and dose-dependent. Cells cycle arrested at Go and G1 phase. ART could depress the migration ability of Tea8113 cells. High-close ART could up-regulate the RECK expression. Conclusion ART could inhibit proliferation of Tca8113 cells in vitro, and possibly depressed migration ability of cells by up-regulating RECK expression.

关 键 词：青蒿琥酯 TCA8113细胞 RECK 抑制 

分 类 号：R285[医药卫生—中药学]