甲型肝炎患者PBMC中HAV-Ag的APAAP检测法及HAV-Ag检出的意义  

Significance and detection of HAV-Ag of PBMC in hepatitis A patients by means of APAAP

作　　者：郝春秋[1] 周永兴[1] 闫荣[1] 陈龙邦[2] 

机构地区：[1]第四军医大学唐都医院感染科,陕西西安710038 [2]南京军区南京总医院肿瘤科,江苏南京210000

出　　处：《第四军医大学学报》2000年第7期808-810,共3页Journal of the Fourth Military Medical University

摘　　要：目的　建立检测甲肝患者外周血单个核细胞(PBMC)中甲肝病毒抗原 (HAV- Ag)的 APAAP方法 ,并对5 2例甲肝患者 PBMC中 HAV- Ag进行动态检测 .方法　应用抗 HAV- Mc Ab,羊抗鼠 Ig G及 APAAP建立了三层重叠的APAAP法 .对 5 2例甲肝患者急性期及恢复期 PBMC进行动态检测 ,对 HAV- Ag阳性率及平均阳性细胞百分率及其与甲肝病程的相关关系进行了分析 .结果　替代试验及阻断试验证明本方法特异性及敏感性好 ,结果可靠 .甲肝患者急性期PBMC中 HAV- Ag阳性率 (5 4.7% )及阳性细胞百分率[(14.2± 8.4) % ]明显高于恢复期 [6 .45 % ,P<0 .0 5 ;(1.8±0 .8) % ,P<0 .0 1];随着病程的延长 ,HAV- Ag阳性率及阳性细胞百分率逐渐下降 ,与病程存在显著负相关 (r=- 0 .99,P<0 .0 0 1;r=- 0 .98,P <0 .0 0 1) .结论　在甲肝病发过程中 ,患者 PBMC中有 HAV- Ag一过性出现 ,提示 HAV一过性侵犯了 PBMC.AIM To establish a method of APAAP to detect HAV-Ag of PBMC in hepatitis A (HA) patients. METHODS APAAP immunohistochemistry technique was established by using anti-HAV-McAb, goat-anti-mouse IgG and APAAP. Dynamic detection of HAV-Ag of PBMC in 52 HA patients was made in acute phase and convalescence. Correlation of positive rate of HAV-Ag, average positive cells percentage of HAV-Ag and course of HA was analyzed. RESULTS Technique examination indicated that the method had good sensitivity and speciality. In acute phase of HA, positive rate (54.7%) and average positive cells percentage [(14.2±8.4)%] of HAV-Ag of PBMC were much higher than those in convalescence [6.45%, P <0.05; (1.8± 0.8 )%, P <0.01]. With HA patients' recovery, positive rate and average positive cells percentage descended gradually, having strong negative correlation with the course of HA ( r =-0.99, P <0.001; r =-0.98, P <0.001). CONCLUSION During the occurrence of HA, HAV-Ag was detected temporarily from PBMC, and this indicates that HAV may invade PBMC temporarily in the same time.

关 键 词：甲型肝炎 外周血意外核细胞 甲肝病毒抗原 APAAP 

分 类 号：R512.61[医药卫生—内科学]