人细小病毒B19-VP2-IgM的检测及其应用价值的研究  

Detection of human parvovirus B19 major capsid protein IgM and its clinical applying value

作　　者：曹玉红[1] 许东亮[1] 张国成[1] 孙新[1] 李飚[1] 

机构地区：[1]第四军医大学西京医院儿科,陕西西安710033

出　　处：《第四军医大学学报》2000年第7期S170-S172,共3页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金!资助项目 ( 3 9870 0 2 1)

摘　　要：目的　评估 EL ISA法检测抗人细小病毒 B19主要衣壳蛋白 Ig M(B19- VP2 - Ig M)诊断 B19病毒感染的价值 ;并初步了解 B19病毒感染临床表现多样性 .方法　对 5 5例小儿常见疾病包括急性再生障碍性贫血 (AA) 10例 .特发性血小板减少性紫癜 (ITP) 10例、急性淋巴细胞白血病 (AL L ) 15例、幼年类风湿关节炎 (JRA) 12例、过敏性紫癜 (SHP) 8例患者血清进行 B19- VP2 - Ig M检测 ;并对上述标本用巢式 PCR检测 B19- DNA作为对照研究 .结果　 1EL ISA法检测 B19-VP2 - Ig M特异性高 ,稳定性好 . 2 B19- VP2 - Ig M总阳性率为10 / 5 5 (18.2 % ) ,其中 AA,ITP,AL L,JRA,SHP阳性率分别为 :2 / 10 (2 0 .0 % ) ,3/ 10 (30 .0 % ) ,2 / 15 (13.3% ) ,2 / 12(16 .7% ) ,1/ 8(12 .5 % ) . 3B19- DNA总阳性率为 17/ 5 5(30 .9% ) ,其中 AA,ITP,AL L ,JRA,SHP阳性率分别为 :3/ 10 (30 . 0 % ) ,4/ 10 (40 . 0 % ) ,4/ 15 (2 6 . 7% ) ,4/ 12(33.3% ) ,2 / 8(2 5 .0 % ) . 4B19- VP2 - Ig M阳性率略小于B19- DNA阳性率 ,但统计学检验无显著差异 (P >0 .0 5 ) .结论　 1EL ISA法检测 B19- VP2 - Ig M简便快速 ,特异性高 ,适用于 B19病毒感染早期诊断 . 2 B19病毒感染临床表现多种多样 ,与小儿 AA,ITP,AL L,JRA,SHP关系密?AIM To study the value of enzyme linked immunosorbent assay (ELISA) in detecting human parvovirus B19 major capsid protein IgM (B19-VP2-IgM) for the diagnosis of B19 infection, and understand the diversity of clinical forms of presentation of B19 infection. METHODS ELISA was employed to detect the B19-VP2-IgM in the serum of 55 children, of whom 10 were with acute aplastic anemia (AA), 10 with idiopathic thrombocytopenic purpura (ITP), 15 with acute lymphocytic leukemia (ALL), 12 with juvenile rheumatoid arthritis (JRA) and 8 with anaphylactoid purpura (Schonlein-Henoch purpura, SHP). A nested polymerase chain reaction (nPCR) assay detecting B19-DNA was used as a control study. RESULTS ① ELISA used to detect B19-VP2-IgM had high specificity and good stability . ② Ten of 55 patients were positive for B19-VP2-IgM (18.2%), of whom 2 were with AA (20%), 3 were ITP (30%), 2 were ALL ( 13.3% ), 2 were JRA (16.7%), and 1 was SHP (12.5%) . ③ The total positive rate of B19-DNA was 17/55 (30.9%) , and the positive rates of AA, ITP, ALL, JRA, SHP were 3/10 (30%),4/10(40%),4/15(26.7%), 4/12 (33.3%) , 2/8 (25%), respectively. ④ The positive rate of B19-VP2-IgM was slightly lower than that of B19-DNA , but there was no significant difference ( P >0.05). CONCLUSION ① The detection of B19-VP2-IgM by ELISA is rapid, easy, special, and can be used for the early diagnosis of B19 infection. ② B19 infection might be closely related to AA, ITP, ALL, JRA and SHP, and the diversity of clinical forms of presentation of B19 infection was well documented. ③ the value of ELISA detecting B19-VP2-IgM for the diagnosis of B19 infection is similar to that of nPCR detecting B19-DNA.

关 键 词：ELISA 聚合酶链反应 人细小病毒B19 免疫球蛋白 

分 类 号：R373[医药卫生—病原生物学]