bFGF和IGF-1细胞生长因子对欧洲鳗鲡胸鳍传代细胞生长的影响  

Growth Factors,bFGF and IFG-1,in Pectoral Fin Passage Cells of Anguilla anguilla

作　　者：毛凝[1] 郑在予[2] 陈少莺[1] 

机构地区：[1]福建省农业科学院畜牧兽医研究所,福建福州350013 [2]福建省农业科学院生物技术研究所,福建福州350003

出　　处：《福建农业学报》2012年第6期578-582,共5页Fujian Journal of Agricultural Sciences

基　　金：福建省科技创新平台建设项目(2009N2003);福建省科技计划项目--省属公益类科研院所基本科研专项(2011R1101025-5)

摘　　要：为建立一种欧洲鳗鲡病毒性疾病分离、鉴定的细胞模型,深入了解鳗鲡病毒性疾病流行与传播的情况,本试验在欧洲鳗鲡胸鳍传代细胞体外培养的最适条件下,通过添加碱性成纤维细胞生长因子(bFGF)和胰岛素样生长因子1(IGF-1)等可能的传代细胞促长因子,并在限定的时间内收集细胞、进行细胞计数、绘制胸鳍细胞的生长曲线,分析其对欧洲鳗鲡胸鳍细胞生长速率的影响。试验结果提示:在10%FBS L-15全价培养基的条件下,bFGF对欧洲胸鳍细胞的生长有明显的促进作用,最佳效应浓度为10μ.L-1;IGF-1(5～50μg.L-1)不利于欧洲鳗鲡胸鳍细胞的生长。To establish a model for cell isolation and identification, as well as to gain an in-depth understanding of the viral diseases in Anguilla anguilla, the roles played by the growth factors, bFGF and IFG-1, on the proliferation of the eel＇s pectoral fin cells were studied. Under in vitro conditions, the cells were collected, counted and growth curves constructed. On the L-15 medium containing 10% FBS, bFGF showed a significant evidence in promoting the growth of the pectoral fin cells. The cellular growth peaked at bFGF concentration of 10 μg · L^- 1. On the other hand, IGF-1 in the concentrations from 5 μg · L-1 to 50 μg ·L-1 did not show any apparent effect on the growth. Therefore, a culture medium formulation for effective proliferation of the A. anguilla pectoral fin cells was obtained. This would, in turn, aid future studies on the pathogen＇s isolation, identification and vaccine preparation.

关 键 词：欧洲鳗鲡 细胞培养 生长因子 

分 类 号：S852[农业科学—基础兽医学]