抗Ia单克隆抗体促进异基因皮肤移植耐受  

Study on the effect of anti-Ia monoclonal antibody in induction of transplantation tolerance to the allogeneic skin graft

作　　者：黄宏思[1] 郝洁[1] 谢蜀生[1] 

机构地区：[1]北京大学基础医学院免疫学系,北京100083

出　　处：《北京医科大学学报》2000年第4期340-342,共3页Journal of Peking University(Health Sciences)

基　　金：国家自然科学基金重点项目!(39830340)资助

摘　　要：目的 :探索抗Ia单克隆抗体促进“细胞 +环磷酰胺 (cyclophosphamide,CP)”系统诱导移植耐受的作用及其机制。方法 :BALB/C(H 2 d)小鼠经尾静脉注入C57BL/ 6 (H 2 b,B6 )小鼠脾细胞 ,48h后腹腔注射CP ,接着两天分别经尾静脉注入抗小鼠Ia单克隆抗体 5 0 0 μg ,然后进行皮肤移植 ,并于耐受 30d对受体小鼠作混合淋巴细胞反应(mixedlymphocytereaction ,MLR)、迟发型超敏反应 (delayedtypehypersensitivity ,DTH)等耐受状态的检查。 结果 :B6小鼠的皮肤移植物在耐受BALB/C小鼠中存活期特异性延长 ,MLR和DTH检查证明BALB/C小鼠对B6小鼠的脾细胞产生特异性耐受 ,对无关第 3者KM小鼠的脾细胞仍表现出强烈的免疫反应。机制研究发现 ,克隆不应答 (anergy)和抑制细胞在耐受中起作用。结论 :抗Ia单克隆抗体可明显促进“细胞 +CP”诱导的异基因皮肤移植耐受。Objective: To investigate the effect and mechanisms of anti Ia monoclonal antibody (McAb) on tolerance induction by using the “cells followed by CP” system. Methods: BALB/C mice (H 2 d) received an injection of allogeneic spleen cells from C 57 BL/6 (B6) mice (H 2 b) via the tail vein, followed by an intraperioneal injection of cyclophosphamide (CP) 48 hours later. In the following 2 days the mice were intraperioneally given anti Ia McAb 500 μg·d -1 , then grafted with allogeneic skin from B6 mice to monitor the survival of the skin grafts. 30 days after induction of tolerance the MLR and DTH of tolerance BALB/C mice were examined to conform the tolerance status. Results: The survival time of allogeneic skin grafts in the tolerant BALB/C mice was prolonged significantly. The results of MLR and DTH indicated that BALB/C mice were tolerant to the spleen cells of B6, but not KM mice. The results of mechanism study suggested that the clonal anergy and suppressor cells exerted the effect on the tolerance. Conclusion: Anti Ia McAb is able to improve the tolerance of allogeneic skin graft induced by “cells followed by CP” system. Clonal anergy and suppressor cells are main mechanisms of the tolerance.

关 键 词：移植耐受 皮肤移植 单克隆抗体 药理学 

分 类 号：R622.1[医药卫生—整形外科] R392.4[医药卫生—外科学]