人参皂甙Rg3对胃癌细胞株BGC-823增殖凋亡的影响  

Effects of ginsenoside-Rg3 on cell proliferation and apoptosis in human gastric cancer cell line BGC-823

作　　者：高川[1] 张明亮[1] 

机构地区：[1]南华大学附属第二医院消化内科,衡阳421001

出　　处：《浙江医学》2012年第14期1184-1187,共4页Zhejiang Medical Journal

摘　　要：目的研究人参皂甙Rg3对胃癌细胞株BGC-823增殖凋亡及其对凋亡基因p53、S100A4表达的影响。方法以62．5—500ug／m1人参皂甙Rg3作用后，通过MTT比色法检测细胞增殖抑制率、AO—EB双染法结合荧光显微镜观察细胞凋亡形态学改变、RT—PCR检测p53、S100A4基因的表达情况。结果BGC-823细胞在62．5—500μg／ml人参皂甙Rg3作用后，细胞抑制率逐渐增加；Rg3处理细胞48h的IC50为250ug／ml，BGC-823细胞出现染色质凝集、核片段化、凋亡小体等凋亡形态学特征；RT-PCR结果显示：人参皂甙Rg3能显著上调p53mRNA表达下调S100A4mRNA表达，且呈浓度依赖性。结论人参皂甙Rg3能显著抑制胃癌细胞株BGC-823细胞的生长，其抗肿瘤机制可能与上调p53基因，下调S100A基因的表达有关。Objective To investigate the effects of ginsenoside-Rg3 on cell proliferation and apoptosis in human gastric cancer cell line BGC-823. Methods The inhibition rate of gastric cancer BGC-823 cells was measured by MTT,cell apoptosis rate were determined by AO-EB staining and the expressions of p53 and S100A4 mRNAs were detected by RT-PCR. Results MTT assay showed that cell proliferation inhibition rates increased with the increasing ginsenoside-Rg3 concentrations from 62.5 to 500 μg/ml; the IC50 was 250μg/ml Rg3 after treatment for 48h. The cells treated with 250μg/ml Rg3 for 48h showed morpho- logical characteristics of apoptosis with the condensed chromatin and the nuclear fragmentation, the apoptotic body and brighter green fluorescent in cells. RT-PCR illustrated that ginsenoside-Rg3 significantly up-regulated the expression of p53 mRNA; whereas the expression of S100A4 mRNA was down-regulated with a concentration-dependent manner. Conclusion Ginseno- side-Rg3 can significantly inhibit the growth of BGC-823 cells, which may be related to the cell apoptosis induction, cell cycle regulation, up-regulation of p53 mRNA and down-regulation of S100A4mRNA expression.

关 键 词：人参皂甙RG3 细胞凋亡 P53 S100A4 

分 类 号：R735.2[医药卫生—肿瘤]