抗心磷脂抗体阳性脑梗死与HLA—DQB1基因多态性关系的研究  

DQB1 gene polymorphism in patients of cerebral infarction with positive anticardiolipin antibody

作　　者：王恩[1] 黄勤 金笑平[1] 朱敏 周美英 李俊 周元林[1] 

机构地区：[1]台州医院神经内科,317000 [2]台州医院中心实验室,317000 [3]台州医院免疫室,317000 [4]台州医院消化内科,317000

出　　处：《浙江医学》2012年第13期1126-1128,1139,共4页Zhejiang Medical Journal

摘　　要：目的探讨抗心磷脂抗体（ACL）阳性脑梗死与人类白细胞抗原DQB1（HLA—DQB1）等位基因多态性的关系。方法采用聚合酶链反应一限制性片段长度方法（PCR—RFLP）．分析150例脑梗死患者[观察组，其中ACL阳性36例，ACL阴性114例】的基因型．并以96名健康体检者为对照占结果（1）ACL阳性率在观察组中明显高于对照组，差异有统计学意义（P〈0．05）；（2）观察组中，DQB1。0303等位基因频率为38．67％（116／300）．高于对照组的29．17％（56／192），差异有统计学意义（P〈0．05）；（3）观察组中，ACL阳性脑梗死DQB1。0303等位基因频率为52．78％（38／72），明显高于ACL阴性脑梗死的34121％（78／228），差异有统计学意义（P〈001）。DQB1。0303等位基因频率与ACL阳性率呈正相关．相对危险率（RR）值为2．15（P=0．00）。结论DQB1。0303可能是ACL阳性脑梗死的遗传易感基因。Objective To investigate the relationship between anticardiolipin （ACL） antibody and soluble human leukocyte antigen （HLA） DQB1 gene polymorphism in patients with cerebral infarction. Methods Polymerase chain reaction-restrictive fragment length polymorphism （PCR-RFLP） was used to type HLA-DQB1 alleles in 36 patients of cerebral infarction with positive ACL, 114 patients with negative ACL and 96 healthy subjects as controls. Results The positive rate of ACL in cerebral infarction patients was higher than that in control group （P〈0.01）. The frequency of DQB1＊0303 in patients of cerebral infarction （38.67%, 116/300 ） was higher than that in control group （ 29.17%, 56/192, P〈0.05）. The frequency of DQBI＊0303 was significantly higher in patients with ACL positive （52.78% 38/72） than that in ACL negative patients （P〈0.01）. The frequency of DQB1＊0303 was posi- tively associated with ACL positive rate in cerebral infarction patients （ RR=2.15, P=0.00）. Conclusion The results suggest that DQB1＊0303 may be associated with ACL（ ＋ ） cerebral infarction.

关 键 词：抗体抗心脂脑梗死HLA—DQ抗原聚合酶链反应 多态性限制性片段长度 

分 类 号：R714.21[医药卫生—妇产科学]