苦参凝集素蛋白基因转化水稻的研究  

Studies on Transformation of SFL Gene into Rice

作　　者：杜平平[1,2] 黄兴奇[1] 李定琴[1] 余腾琼[1] 程在全[1] 

机构地区：[1]云南省农业科学院生物技术与种质资源研究所,昆明650223 [2]云南农业大学农学与生物技术学院,昆明650201

出　　处：《西北植物学报》2012年第8期1518-1524,共7页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家转基因专项(2009ZX08001-007B);国家转基因专项子课题(2011ZX08001-001;2011ZX08001-002);云南省科技项目(2010BB004)

摘　　要：以水稻杂交品种‘云资粳41号’为受体材料,通过农杆菌介导法将苦参凝集素蛋白基因(SFL)导入水稻细胞,采用氯酚红法和PCR检测外源基因是否整合到水稻基因组中。结果显示:外源基因成功转入水稻基因组,并获得一批转基因水稻植株;转基因植株叶片离体接种稻瘟病菌的检测结果显示,转基因植株与对照(非转基因植株)相比有明显的抗性,证明SFL基因在水稻中得到表达。研究表明,基于SFL基因所具备的广谱抗菌作用,可以预期所得转基因水稻植株很可能对水稻的多种病原菌具有良好的抗性,为选育新的抗稻瘟病水稻新品种以及拓宽栽培稻抗病遗传基础增加抗稻瘟病基因奠定了基础。In order to increase rice resistance to blast with more exogenous genes,Sophora flavescens lectin （SFL） encoding lectin which can prohibit the growth of the blast fungus in vitro was introduced into rice cultivar ＇Yunzijing41＇ by Agrobacteriurn-mediated transformation method. A lot of transgenic plants were selected by chlorophenol-red （CPR） assay and confirmed by PCR. Compared with non-transgenic rice,the transgenic plants showed high resistance to Piricularia oryzae Cav. SFL has broad-spectrum antimicrobial activity,so the transgenic rice may has good resistance to variety of pathogenic bacteria. This experiment was laid the foundation for researching on breeding of blast resistance in rice varieties and broaden the disease-resistant genetic and increase rice blast resistance gene in future.

关 键 词：苦参凝集素蛋白基因 水稻 遗传转化 

分 类 号：Q789[生物学—分子生物学]