七氟烷短时吸入对新生大鼠海马caspase-3活性和认知功能发育的影响  

Effects of short-term exposure to sevoflurane on cognitive function development and hippocampal caspase-3 activation in neonatal rats

作　　者：卢国林[1] 张素晶[1] 陈小琳[1] 宋一一[2] 

机构地区：[1]福建省妇幼保健院麻醉科,福建福州350001 [2]福建省妇幼保健院妇科,福建福州350001

出　　处：《中国新药与临床杂志》2012年第8期458-461,共4页Chinese Journal of New Drugs and Clinical Remedies

摘　　要：目的探讨七氟烷短时吸入对新生大鼠海马caspase-3活性和认知功能发育的影响。方法 7日龄新生SD大鼠64只,随机分为A、B、C、D 4组(n=16):A组吸入40%氧气1 h,B、C、D组分别吸入1.0%、2.0%、4.0%七氟烷1 h。各组随机取8只大鼠,于吸入七氟烷后24 h采用流式细胞仪定量分析海马caspase-3的活性,观察海马神经细胞凋亡情况;各组余8只大鼠于7 wk采用Morris水迷宫测试其认知功能。结果 B、C、D组Morris水迷宫测试成绩低于A组,而且随着吸入七氟烷浓度的增加,逃避潜伏期和探索时间逐渐延长,差异有统计学意义(均P<0.01);吸入1.0%、2.0%、4.0%七氟烷大鼠海马神经细胞凋亡率分别为(31.0±2.7)%、(52.8±4.9)%、(71.4±3.2)%,均高于A组(1.9±0.8)%,而且随着吸入七氟烷浓度的增加,神经细胞凋亡率逐渐增加,差异有统计学意义(均P<0.01)。结论七氟烷短时吸入导致海马神经细胞凋亡,并阻抑新生大鼠认知功能发育。AIM To investigate the effects of short-term exposure to sevoflurane on cognitive function development and hippocampal caspase-3 activation in neonatal rats. METHODS Sixty-four 7-day-old neonatal Sprague Dawley rats were randomly divided into four groups （16 rats in each group）. Rats in the group A, B, C and D were respectively inhaled with 40% oxygen, 1.0%, 2.0% and 4.0% sevoflurane for 1 hour. Eight rats in each group were randomly subjected to hidden platform trails and probe trails in Morris water maze to access cognitive function at age of 7 weeks after anesthetic exposure to sevoflurane. Twenty-four hours after completion of sevoflurane exposure, the neurodegeneration and the activation of caspase-3 in hippocampus were analyzed by flow cytometry. RESULTS Latencies and probe times of group B, C and D were significantly longer than those of group A, which raising with the increasing concentrations of sevoflurane exposure （P 〈 0.01）. The ratios of neurodegeneration in hippocampus of group B, C and D were （31.0 ± 2.7） %, （52.8± 4.9） %, （71.4 ± 3.2） %, and were significantly higher than those of group A. The ratios of neurodegeneration were in accordance with the increasing concentrations of sevoflurane （P 〈 0.01）. CONCLUSION Short-term exposure to sevoflurane may interfere the development of cognitive function and induce hippocampal neurodegeneration in neonatal rats.

关 键 词：七氟烷 CASPASE-3 海马 认知功能 大鼠 

分 类 号：R971.2[医药卫生—药品]