维生素C对建鲤肠上皮细胞增殖分化的影响  

Effects of Vitamin C on Proliferation and Differentiation of Intestinal Epithelial Cells in Jian Carp

作　　者：刘扬[1,2,3] 池磊 冯琳[1,2,3] 姜俊[2,3] 周小秋[1,2,3] 

机构地区：[1]四川农业大学动物营养研究所,四川雅安625014 [2]鱼类营养与安全生产四川省高校重点实验室,四川雅安625014 [3]动物抗病营养教育部重点实验室,四川雅安625014 [4]成都三旺农牧股份有限公司,四川新津621430

出　　处：《动物医学进展》2012年第8期41-46,共6页Progress In Veterinary Medicine

基　　金：四川省教育厅重点项目(2009ZA070)

摘　　要：为研究维生素C对原代培养建鲤肠道上皮细胞(IECs)增殖分化、结构和功能的影响,原代培养建鲤肠道上皮细胞72h,换用含维生素C浓度分别为0、4、10、16、22、28mg/L的DMEM培养液,继续培养72h后,观察细胞培养特性和测定细胞蛋白含量、钠钾ATP酶(Na+、K+-ATP酶)、谷草转氨酶(GOT)和谷丙转氨酶(GPT)活性。结果显示,未添加维生素C的建鲤IECs存活数量少,细胞集落较小;添加不同浓度维生素C的试验组建鲤,其细胞贴壁数量明显增多,细胞集落较大并有成片单层细胞,不同试验浓度组间差异不显著;添加不同浓度维生素C的试验组建鲤IECs,MTT OD值、碱性磷酸酶(AKP)、谷丙转氨酶、谷草转氨酶,Na+、K+-ATP酶活性和IECs蛋白含量均显著提高(P<0.05),且培养液中乳酸脱氢酶(LDH)活性显著降低(P<0.05);10mg/L维生素C浓度试验组建鲤IECs的碱性磷酸酶、谷草转氨酶、谷丙转氨酶的活性可增加到最大值,16mg/L维生素C浓度试验组建鲤IECs的Na+、K+-ATP酶活性和蛋白含量增加到最大值。提示适当添加维生素C可以促进建鲤肠道上皮细胞增殖分化,改善细胞膜结构的完整性,增强肠上皮细胞的功能和氨基酸代谢,提高细胞蛋白质沉积。To investigate the effects of vitamin C on the proliferation, differentiation, structure, and func- tion of carp intestinal epithelial cells in primary culture, the carp intestinal epithelial cells （IEC） in primary culture were cultured in 24-well plate after 72 h, then changed into the DMEM culture medium which con- tained 0, 4, 10, 16, 22, and 28 mg/L vitamin C, respectively. The samples were collected to measure the indices after 72 h vitamin C culture. The results showed that vitamin C deficiency lead to less IEC survival number and small colony; adding vitamin C, adherent cell number increased significantly, and IEC format- ted the large colony and monolayer, and the MTT OD, alkaline phosphatase （AKP）, alanine transaminase （GPT）, glutamic-oxalacetic transaminase （GOT）, Na＋, K＋-ATPase activities, and protein contents in IEC significantly improved （P〈0.05）, lactate dehydrogenase （LDH） in culture medium reduced signifi- cantly （P〈0.05）, although there were the differences among the diferent concentrations of vitamin C, eg. 10 mg/L and 16 mg/L. In summary, vitamin C can promote proliferation and differentiation of carp IEC, improve the structure of the cell membrane integrity, enhance intestinal cell function and metabolism of a- mino acids, increase cell protein deposition.

关 键 词：建鲤 维生素C 肠上皮细胞 增殖 分化 

分 类 号：Q564[生物学—生物化学] Q813.1