高能量膳食诱导食蟹猴2型糖尿病模型的研究  

Study on the Model of T2DM of Cynomolgus Induced by High-energy Diet

作　　者：张艳春 靳丽莎[2] 高丽华[3] 彭白露 何红[4] 季芳[2] 郝香芬[2] 张秀娟 唐湘北[2] 饶军华 刘晓明[2] 

机构地区：[1]广东蓝岛生物技术有限公司,广东广州510555 [2]广东省昆虫研究所,广东广州510260 [3]吉林大学第二医院心血管内科,吉林长春130041 [4]深圳市福田区慢性病防治院,广东深圳518048

出　　处：《动物医学进展》2012年第8期47-52,共6页Progress In Veterinary Medicine

基　　金：广州市科技计划项目大型实验动物国际认证及外包服务项目(2009A1-E051);广东省林业局实验猴繁育关键技术研究及产业示范项目(2009KJCX016);国家"十二五"人类重大疾病灵长类动物模型资源平台的建设(2011ZX093073-024)

摘　　要：为构建食蟹猴T2DM进程序列模型,将36只食蟹猴分为4组,分别采用标准基础膳食和高能量膳食(高糖膳食、高脂膳食和高糖高脂膳食)进行诱导,每6个月测量体重、血液生化指标、口服糖耐量和尿检试验。结果显示,高能量膳食组的血糖、血脂水平与初期相比,都有显著升高。其中高糖组的T2DM发病进程较快,高糖高脂组的发病进程相对较慢,但发病进程大致相同。在T2DM临床期之前,不同膳食组在相同发病阶段血脂水平存在差异,至临床期差异消失。根据结果可得,不同膳食成分的高能量膳食均能诱发食蟹猴T2DM进程序列模型,该序列模型是模拟自发型糖尿病发病机理构建的较理想T2DM动物模型。A total 36 cynomolgus were used to induce the process model of type 2 diabetes(T2DM) by feeding with standard basic feed,high-sugar,high-fat and both high-sugar & fat diet,respectively,for 24 months.The development and progression of T2DM were monitored through body weight,blood biochemical index,oral glucose tolerance and urinalysis test every six months.The results showed that the level of fasting plasma glucose(FPG),triglycercide(TG),cholesterin(CHO),high density lipoprotein cholesterin(HDL) and low density lipoprotein cholesterin(LDL) of cynomolgus in high-energy groups were significantly higher than that in the initial individuals.The time when high-sugar objects had clinical symptoms was earlier than that in other high-energy ones,there were still no sample in clinical phase of the both high-sugar & fat groups.The levels of serum lipids for different dietary groups were different in the same phase,but they were basically the same in clinical phases.In conclusion,this method to induce the T2DM process model of cynomolgus by high-energy diet was feasible.

关 键 词：高能量膳食 食蟹猴 2型糖尿病模型 发病进程 

分 类 号：R587.1[医药卫生—内分泌] R589.2[医药卫生—内科学]