小檗碱抑制HCT116细胞生长与Wnt/β-catenin信号的关系研究  

Study on the correlation between the anti-proliferation effect of berberine on HCT116 cells and Wnt/β-catenin signaling

作　　者：杨秋珺[1,2] 周龙洋[1] 刘映孜[1] 牟钰钦[1] 吴柯[1] 周岐新[1] 罗进勇[2] 何百成[1,2] 

机构地区：[1]重庆医科大学药理学教研室,重庆400016 [2]重庆医科大学检验学院分子医学实验室,重庆400016

出　　处：《中国药理学通报》2012年第9期1234-1238,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81071462);重庆市自然科学基金资助项目(No 2011BB5129)

摘　　要：目的研究小檗碱对结肠癌细胞的增殖抑制作用与Wnt/β-catenin信号的关系。方法结晶紫染色法检测小檗碱对HCT116细胞增殖的抑制作用,采用流式分析及West-ern blot研究小檗碱诱导HCT116细胞凋亡;Western blot检测β-catenin蛋白表达、RT-PCR法检测β-catenin mRNA表达,确定小檗碱对其表达的影响;采用外源性过表达β-cate-nin研究小檗碱抑制HCT116细胞增殖与β-catenin的关系。结果小檗碱处理组细胞较对照组细胞增殖明显受到抑制,并诱导HCT116细胞凋亡。小檗碱处理组全细胞、胞质及核内β-catenin蛋白水平均明显较对照组低。外源性过表达β-catenin能减弱小檗碱对HCT116细胞增殖的抑制作用。小檗碱处理组β-catenin mRNA表达明显受到抑制。结论小檗碱能抑制HCT116细胞增殖并抑制Wnt/β-catenin信号转导,其机制可能与下调β-catenin mRNA表达有关。Abstract： Aim To investigate the anti-proliferation effect of the correlation between berberine on HCT116 cells and Wnt/β-catenin signaling transduction. Methods Crystal violet staining was employed to detect the anti-proliferation effect of berberine on HCT116 cells. The apoptosis induced by berberine was detected by fluorescence activated cell sorting and Western blot. The β-catenin expression was measured by Western blot and reverse transcription and polymerase chain reaction （RT-PCT）. With exogenous expression of β- catenin, the role of β-catenin in berberine induced pro- liferation inhibitory effect on HCT116 cells was explored. Results Berberine could inhibit the prolifera-tion and induce apoptosis in HCT116 cells. The expression of β-catenin protein in whole cell, cytoplasm and nucleus was inhibited when treated HCT116 cells with different concentrations of berberine. So was the mRNA expression level of β-catenin. Exogenous expression of β-catenin could attenuate the anti-proliferation effect of berberine on HCT116 cells. Conclusions Berberine can inhibit the proliferation of HCT116 cells and Wnt/β-catenin signaling transduction. This effect may be mediated at least by down regulating the mRNA expression of β-catenin.

关 键 词：小檗碱 HCT116细胞 增殖抑制 凋亡 Wnt/β-cate-nin 信号通路 

分 类 号：R284.1[医药卫生—中药学] R329.24[医药卫生—中医学]