RP-HPLC法测定川牛膝中杯苋甾酮的含量  

RP-HPLC Determination of Cyasterone in Radix Cyathulae

作　　者：陈幸[1] 黎万寿[1] 朱久武[2] 崔红梅[1] 

机构地区：[1]四川省中药研究所,成都610041 [2]成都中医药大学

出　　处：《药物分析杂志》2000年第4期234-237,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：国家"九五"攻关课题!No.96-903-02-04

摘　　要：目的 :首次测定川牛膝中主要成分杯苋甾酮的含量。方法 :采用反相高效液相色谱法。色谱柱YWGC18,流动相为甲醇 -水 (35∶6 5 ) ,流速 1mL·min-1,检测波长 2 43nm。结果 :杯苋甾酮在 0 0 34～ 0 2 7μg范围内线性良好。杯苋甾酮的平均加样回收率为 98 35 % ,RSD为± 1 6 % (n =5 )。结论 :本方法简便、快捷 。Objective:To determine cyasterone in Radix Cyathulae.Methods:The separation was performed on YWG C 18 column with methanol-water(35∶65) as mobile phase.The flow rate was 1 mL·min -1 .The wavelength for measurement was 243 nm.Results:According to the selected chromatographic conditions,the calibration case of cyasterone was linear in the range of 0 034～0 27 μg.The average recoveries of cyasterone was (98 35±1 6)%, n =5.Conclusion:The method is easy and rapid,with good reproducibility.

关 键 词：川牛膝 杯苋甾酮 反相高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学] R282.71[医药卫生—中医学]