污泥龄对活性污泥处理微量磺胺类药物的影响  

Impact of sludge residence time on the relative biodegradation and biosorption of sulfonamide antibiotics in activated sludge

作　　者：杨程[1] 郭劲松[1] Belinda S.M.Sturm Rachael F.Lane Craig D.Adams Ray E.Carter 

机构地区：[1]重庆大学城市建设与环境工程学院,重庆400045 [2]关国堪萨斯大学土木建筑与环境工程学院,美国堪萨斯州劳伦斯66045

出　　处：《重庆大学学报（自然科学版）》2012年第6期63-71,共9页Journal of Chongqing University

基　　金：国家水专项资助项目(2009ZX07104-002;2009ZX07104-003)

摘　　要：为了研究污泥龄对活性污泥系统处理微量磺胺类药物(5μg/L)的影响,共运行了4个试验室规模(3L)的序批式活性污泥反应器(SBR),其污泥龄分别为2、8、14、20d。批次摇瓶试验通过设置3个工况(正常运行,加入生物抑制剂,无微生物)来讨论在1个运行周期(8h)中对浓度为5μg/L磺胺甲恶唑的吸附作用、生物降解作用和挥发损失。试验结果显示对磺胺甲恶唑的总去除量为2.14±0.60μg/g SS,吸附作用占总去除量的63%;磺胺嘧啶为1.14±0.63μg/g SS,83%;磺胺间二甲氧为2.33±0.67μg/g SS,35%;磺胺甲基嘧啶为2.45±0.85μg/g SS,55%;磺胺类药物的去除效果与污泥的污泥龄有着非常显著的关系(p<0.02)。通过运行加入磺胺甲恶唑(进水5μg/L)的反应器60d,4个反应器对磺胺甲恶唑的平均去除率分别为10%、41%、51%、58%,处理效果随着污泥龄的增加而变好,同时单位污泥去除率随着污泥龄的增加而降低,SRT=2d的反应器由于存在大量的丝状菌,使得单位污泥对磺胺甲恶唑去除率大大高于其他3个反应器。通过分子生物学分析,发现微生物群落结构的变化不大,从而说明了影响磺胺类药物处理效果的主要因素在于更强的吸附能力,更高的污泥浓度。Four sequencing batch reactors（SBR） are operated with sludge residence time（SRT）of 2,8,14,and 20 days to analyze the impact of SRT on removal of sulfanomide（5 μg/L）.In batch experiments,three treatments（active biomass,inhibited biomass,and controls of no biomass） are performed in 100 mL batch reactors in triplicate that simulated one SBR cycle,and are investigated to distinguish biodegradation,biosorption,and volatilization losses,respectively.The results show that biomass removed an average 2.14±0.60 μg/g SS of sulfamethoxazole,1.14±0.63 μg/g SS of sulfadiazine,2.33±0.67 μg/g SS of sulfadimethoxine,2.45±0.85 μg/gSS of sulfamerazine,with 63%,83%,35%,55% of the removal respectively due to sorption.When SBRs（3 L total volume） are spiked continuously with 5 μg/L sulfamethoxazole for 60 days,10%,41%,51%,58% is removed with SRTs of 2d,8d,14d,20d,respectively.Removal increased significantly with SRT,but the normalized mass removed per gram of biomass decreases.Growth of filamentous organisms with a 2-d SRT increases the sorption capacity of this sludge,although biodegradation is the dominant mechanism for removal.Higher biomass concentrations established by longer SRTs are more significant for biodegradation than species diversity,which do not vary with SRT.As wastewater treatment plants implement longer SRTs for nutrient removal,they will also achieve improved removal of some pharmaceuticals.

关 键 词：DGGE活性污泥系统 磺胺类抗生素 磺胺甲恶唑 污泥龄 生物降解 生物吸附 

分 类 号：X703[环境科学与工程—环境工程]