PBDE-47对人神经母细胞瘤细胞凋亡和线粒体膜电位及细胞色素C蛋白表达的影响  

Effect of PBDE-47 on apoptosis,mitochondrial membrane potential and protein expression of cytochrome C in SHSYSY cells

作　　者：吴雪[1] 陈义虎[1] 姜春阳[1] 张舜[1] 张小非[1] 杨露[1] 张成[1] 雷蓉蓉[1] 夏涛[1] 王爱国[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院劳动卫生与环境卫生学系环境与健康教育部重点实验室,湖北武汉430030

出　　处：《环境与健康杂志》2012年第8期675-678,共4页Journal of Environment and Health

基　　金：国家自然科学基金面上项目(81072266;30872081)

摘　　要：目的探讨PBDE-47对人神经母细胞瘤(SH-SY5Y)细胞线粒体膜电位和细胞色素C(Cyt C)蛋白表达的影响及其与细胞凋亡的关系。方法将处于对数生长期的SH-SY5Y细胞分别加入终浓度为0(溶剂对照组)、1、5、10μmol/L的PBDE-47暴露24 h。采用流式细胞术检测细胞凋亡率和线粒体膜电位变化,采用Western blot技术检测胞浆与线粒体细胞色素C蛋白表达的水平。结果与溶剂对照组相比,10μmol/L PBDE-47染毒组SH-SY5Y细胞凋亡率和5、10μmol/L PBDE-47染毒组胞浆内细胞色素C蛋白表达水平均明显升高,10μmol/L PBDE-47染毒组线粒体膜电位和各浓度PBDE-47染毒组线粒体内细胞色素C蛋白表达水平均明显下降,差异均有统计学意义(P<0.05)。且随着PBDE-47染毒浓度的升高,SH-SY5Y细胞凋亡率和胞浆内细胞色素C蛋白表达水平呈升高趋势,线粒体内细胞色素C蛋白表达水平呈下降趋势。结论 PBDE-47可通过引起线粒体膜通透性转运孔开放和细胞色素C的释放介导SH-SY5Y细胞凋亡,从而对SH-SY5Y细胞产生损伤。Objective To explore the effects of PBDE-47 on mitochondrial membrane potential （AXiS＇m） and protein expression level of cytoehrome c （Cyt C） in human neuroblastoma cells （SH-SYSY cells）, and to study the relationship between them and apoptosis. Methods The SH-SYSY cells of logarithmic phase were exposed to final concentrations of 0 （solvent control group）, 1,5,10 μmol/L PBDE-47 for 24 h in vitro. The percentages of apoptosis and the changes of △ψm were analyzed by flow cytometry, and the protein expression levels of cytosolic and mitochondrial Cyt C were detected by Western blot. Results Compared with the solvent control group, the percentage of apoptosis increased significantly （P〈0.05） in 10 μmol/L PBDE-47 treated group,and eytosolic Cyt C protein expression levels increased significantly in 5 and 10 μmol/L PBDE-47 treated groups（P〈0.05）;△ψm decreased（P〈0.05） in 10 μmol/L PBDE-47 treated group,and mitochondrial Cyt C protein expressions in various concentrations of PBDE-47 decreased significantly（P〈0.05 ） compared with those in the solvent control group. As the concentrations of PBDE-47 increased, the apoptosis rates and protein expression level of cytosolic cytochrome C in SH-SYSY cell showed an increasing trend, and the protein expression level of mitochondrial eytochrome C revealed a declining trend. Conclusion PBDE-47 may induce SH-SYSY cells apoptosis by mitochondrial permeability transition pore opening and the release of Cyt C ,therefore it may cause the injury of SH-SYSY cells.

关 键 词：PBDE-47 人神经母细胞瘤细胞 细胞凋亡 线粒体膜电位 细胞色素C 

分 类 号：R994.6[医药卫生—毒理学]