烟草β-胡萝卜素羟化酶基因的克隆与序列分析  

Cloning and Sequence Analysis of β-carotene Hydroxylase Gene in Nicotiana tabacum

作　　者：林世锋[1] 王仁刚[1] 王轶[1] 张吉顺[1] 任学良[1] 

机构地区：[1]贵州省烟草科学研究所,贵阳市金阳新区云潭北路550081

出　　处：《烟草科技》2012年第8期70-74,共5页Tobacco Science & Technology

基　　金：中国烟草总公司重点项目"烤烟育种新技术与新一代烤烟新品种选育研究"(2010-221);贵州省科技厅农业攻关项目"烤烟种质资源鉴定与创新及新品种选育研究"(2011-3047)

摘　　要：为有效调控烟草类胡萝卜素的合成代谢,从普通烟草中克隆到与植物类胡萝卜素合成代谢相关的β-胡萝卜素羟化酶基因BCH,以辣椒BCH基因为探针,通过电子克隆的方法从烟草栽培品种K326中分离出一条编码BCH基因的cDNA序列,暂命名为NtBCH1(GenBank登录号JQ410446)。NtBCH1包含一个完整的开放阅读框,编码309个氨基酸,具备脂肪酸羟化酶超家族保守结构域。序列比对结果表明,NtBCH1蛋白与其他植物的BCH蛋白具有很高的相似性。系统进化树分析表明,NtBCH1与番茄的亲缘关系最近。In order to effectively modulate the anabolism of carotenoid in tobacco, a B-carotene hydroxylase （BCH） gene related to plant carotenoid anabolism was cloned from Nicotiana tabacurn. A cDNA sequence of encoded BCH gene was isolated from tobacco cv. K326 by using in silico cloning technique with pepper BCH gene sequence as a probe and tentatively designated as NtBCH1 （GenBank, JQ410446）. NtBCH1 gene has a complete open reading frame encoding 309 amino acids with a conserved structural domain of FA_hydroxylase superfamily. The sequence comparison shows high homology to other plant BCH proteins of NtBCH1 protein, and the phylogenetic tree analysis indicates the closest genetic relationship between NtBCH1 and Solanum lycopersicum.

关 键 词：普通烟草 β-胡萝卜素羟化酶(BCH) 基因 克隆 序列分析 

分 类 号：S572[农业科学—烟草工业]