烟草青枯病病圃土壤微生物多样性初步分析  

Preliminary Analysis of Soil Microbial Diversity in Tobacco Bacterial Wilt Disease Nursery

作　　者：蔡刘体[1,2] 商胜华[1] 胡重怡[1] 王丽丽[2] 刘艳霞[2] 汪汉成[1] 杨兴明[2] 石俊雄[1] 

机构地区：[1]贵州省烟草科学研究所,贵阳市金阳新区云潭北路550081 [2]南京农业大学资源与环境科学学院,南京市卫岗童卫路1号210095

出　　处：《烟草科技》2012年第8期79-83,共5页Tobacco Science & Technology

基　　金：贵州省烟草专卖局项目"烟草病圃微生物多样性分析及烟草抗性基因研究"(黔烟科2009-15);贵州省科技厅项目"烟草青枯病病原菌天敌的筛选及生防体系研究"[黔科合J字(2010)2087]

摘　　要：为了更好地利用烟草青枯菌拮抗菌防治烟草青枯病,采用微生物保守区域扩增、构建文库、生物信息比对的方法初步分析了烟草青枯病病圃土壤中微生物的多样性。结果显示,烟草青枯病病圃土壤微生物群落中含有伯克霍尔德氏菌(Burkholderia fungorum)、假单胞杆菌属类(Pseudomonas graminis)、黑霉菌(Aspergillus niger)、尖孢镰刀菌属(Fusarium oxysporum)等丰富的细菌类和真菌类微生物,也包括部分不可培养的微生物。土壤中微生物多样性的分析可以为青枯菌拮抗菌应用中拮抗菌在土壤中的定殖、扩繁过程研究提供部分微生物群落信息。To prevent and control tobacco bacterial wilt disease with antagonistic bacterium against Ratstonta solanacearurn, the microbial diversity in soil of tobacco bacterial wilt disease nursery was preliminarily analyzed by means of conservative fragment amplification, construction of library and bio-inforrnation comparison. The results indicated that there existed abundant microbial communities including not only Burkholderia fungorum, Psettdomonas graminis, Aspergillus niger and Fusarium oxysporum, but also some uncultivable bacterium in the soil. The analysis of microbial diversity in the soil could provide some information about microbial community structure for the study on colonization and proliferation process in soil in the application of antagonistic bacterium against Ralstonia solanacearurn.

关 键 词：烟草 土壤 微生物 青枯病 青枯菌 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]