α-玉米赤霉醇对成骨细胞增殖、分化以及OPG和RANKL mRNA表达的影响  

Effects of α-zearalanol on proliferation and differentiation of osteoblast and OPG/RANKL mRNA expression in vitro

作　　者：刘璐[1] 李建宇[2] 万宗明[3] 

机构地区：[1]武警后勤学院化学教研室,天津300162 [2]武警后勤学院药化教研室,天津300162 [3]武警后勤学院药理教研室,天津300162

出　　处：《中国新药杂志》2012年第16期1926-1929,1952,共5页Chinese Journal of New Drugs

基　　金：武警医学院面上项目(WY200908)

摘　　要：目的:探讨α-玉米赤霉醇(α-zearalanol,α-ZAL)对小鼠成骨样细胞MC3T3-E1增殖、分化及护骨素(osteoprotegerin,OPG)和NF-кB受体活化配体(receptor activator of nuclear factor-кB ligand,RANKL)mRNA表达的影响。方法:传代培养小鼠成骨样细胞株MC3T3-E1,采用不同浓度的α-ZAL作用于细胞72 h后,MTT法检测细胞增殖活性,PNPP法检测碱性磷酸酶(alkaline phosphatase,ALP)活性,RT-PCR法检测ALP,OPG及RANKL mRNA的表达水平。结果:10-6～10-12mol·L-1的α-ZAL可显著抑制成骨细胞的增殖(P<0.05);显著增加ALP活性(P<0.05),但不同剂量间存在作用时间差异;并且可显著上调成骨细胞内OPG/RANKL mRNA的比值(P<0.05)。结论:α-ZAL可抑制成骨细胞的增殖、促进其分化,并可通过上调OPG/RANKL mRNA表达比值抑制破骨细胞的形成,有望作为骨质疏松症的治疗药物。Objective： To study the effects of α-zearalanol （α-ZAL） on the proliferation and differentiation of osteoblast and the mRNA levels of osteoprotegerin （OPG） and receptor activator nuclear factor-kappa B ligand （RANKL） , thus to find the candidate for the treatment of osteoporosis. Methods： MC3T3-E1 cells were cultured in different concentrations of α-ZAL for different time respectively in vitro. Cell proliferation was determined by MTT method, and osteoblast differentiation was assessed by ALP activity assay. The mRNA levels of ALP, OPG and RANKL were semi-quantified by RT-PCR. Results： α-ZAL at the concentration of 10^-6-10^-12mool. L^-1 could depress the proliferation of MC3T3-E1 cells （P 〈 0.05） while enhancing ALP activity （P 〈 0.05 ） with significant differences between different dosages at different culture time. α-ZAL also could raise the mRNA levels of OPG/ RANKL ratio （P 〈 0.05）. Conclusion： Suitable concentration of α-ZAL can inhibit osteoblast proliferation, pro- mote osteoblast differentiation of MC3T3-E1 cells and inhibit the formation of osteoclast. Therefore, it would be a promising candidate for the treatment of osteoporosis.

关 键 词：Α-玉米赤霉醇 成骨样细胞 护骨素 碱性磷酸酶 植物雌激素 

分 类 号：R977[医药卫生—药品]