超临界二氧化碳萃取喜树种子中喜树碱  

Optimization of Supercritical CO_2 Extraction of Camptothecin from Camptotheca acuminata Seeds

作　　者：张玉红[1,2] 周志强[1,2] 王洋[3] 

机构地区：[1]东北林业大学森林植物生态学教育部重点实验室,黑龙江哈尔滨150040 [2]东北林业大学林业生物制剂教育部工程研究中心,黑龙江哈尔滨150040 [3]东北林业大学盐碱地生物资源环境研究中心,黑龙江哈尔滨150040

出　　处：《精细化工》2012年第8期757-760,共4页Fine Chemicals

基　　金：国家科技支撑计划项目(2006BAD18B04)~~

摘　　要：用超临界CO2流体萃取技术从喜树种子中提取喜树碱。在CO2流量20 L/h的条件下,探讨了夹带剂、萃取压力、萃取温度和萃取时间对喜树种子中喜树碱提取率的影响。通过正交实验设计验证并确定了超临界CO2萃取喜树种子中喜树碱的最佳工艺条件为:萃取压力25 MPa,萃取温度50℃,萃取时间120 min,夹带剂乙醇体积分数90%。在该条件下,喜树碱平均提取率为76.98%,所得到的萃取物中喜树碱的质量分数达43.68%。Supercritical CO2 fluid extraction technology was used to extract camptothecin from Camptotheca acumirtata seeds. With the precondition of CO2 flux 20 L/h, the effect of different parameters such as extraction time, temperature, extraction pressure and entrainer on the supercfitical carbon dioxide extraction of camptothecin was investigated. The optimum technological conditions were validated by an orthogonal experimental design：extraction pressure 25 MPa, temperature 50 ℃, extraction time 120 min, and entrainer 90% ethanol. Under the optimal conditions, the extraction rates of camptothecin was 76. 98% ,and the mass fraction of camptothecin was 43.68% in the crude extracts.

关 键 词：喜树碱 超临界CO2萃取 喜树种子 夹带剂 催化与分离提纯技术 

分 类 号：TQ420.6[化学工程]