高效液相色谱-荧光检测方法用于单胺氧化酶抑制剂抑制效果的评价  

HPLC-Fluorescence Detection Used for Evaluating the Effect of Monoamine Oxidase Inhibitor

作　　者：王珊珊[1] 马培翔[2] 

机构地区：[1]山东省分析测试中心大型精密仪器重点实验室,山东济南250014 [2]北京理工大学生命科学与技术学院,北京100081

出　　处：《生命科学仪器》2012年第4期17-20,共4页Life Science Instruments

基　　金：十一五科技支撑项目(NO.2006BAK03A07)

摘　　要：单胺氧化酶是一种重要的调节神经递质代谢的黄素蛋白,其抑制剂的研究对治疗抑郁症和许多神经退行性疾病具有重要的意义,建立了一种用HPLC-荧光检测器测定单胺氧化酶抑制剂的新方法。实验中使用的单胺氧化酶来源于Wistar雄性大鼠的鼠肝,以犬脲胺为底物,考查了治疗精神疾病的常用药物帕吉林及氯吉兰对单胺氧化酶的抑制效果。与其它检测方法相比,其操作简单安全,可快速检测药物对单胺氧化酶的抑制效果。Monoamine oxidase （MAO, EC 1.4.3.4） is an important membrane-bound flavoenzyme that can modulate the metabolism of The research of its inhibitor plays an important role in treating depressive disorder and other neurodegenerative diseases. In this study, we established a new method to determine the efficiency of the inhibitor using HPLC- Fluorescence Detector. In this study, the monoamine oxidase was obtained from liver of Wistar male rat, and Kynuramine was used as substrate. We measured the enzyme repression of Pargyline and Clogyline that the commonly medicine used to treat mental disorder. Compared to other methods, this approach manipulates simple and safety, can detect the inhibition efficiency quickly.

关 键 词：单胺氧化酶 犬脲胺 抑制剂 高效液相色谱 荧光检测 

分 类 号：Q255[生物学—细胞生物学]