饥饿小鼠和饱食小鼠肝糖原含量及血糖的比较  

Hungry Mice and Sated Mice Hepatic Glycogen Content and Glucose Comparison

作　　者：罗永会[1] 张翠香[1] 徐春萍[1] 

机构地区：[1]大理学院基础医学院生物化学与分子生物学实验室,云南大理671000

出　　处：《生命科学仪器》2012年第4期25-27,共3页Life Science Instruments

摘　　要：目的:通过对饥饿小鼠和饱食小鼠血糖及肝糖原测定,了解饥饿及饱食对血糖和肝糖原有影响。方法:取体重在25g以上的健康小鼠60只,随机分成两组:饥饿组30只,实验前严格禁食不禁水30h;饱食组30只:自由摄食,饮水。然后采用蒽酮显色法测定小鼠肝糖原,用Folin-吴法测定小鼠血糖。结果:饥饿与饱食进行比较小鼠血糖及肝糖原都表现为明显的降低或升高趋势(即饥饿小鼠血糖及肝糖原降低,饱食小鼠血糖及肝糖原升高),且P<0.05具有统计学意义。结论:饱食后肝糖原增加,饥饿肝糖原逐渐降低;饱食后血糖明显升高,饥饿则血糖明显降低。Objective： Hunger and satiation on mouse Liver glycogen and Blood glucose determination, Awareness of hunger and satiation on blood glucose and glycogen in the original effect.. Method： Taking the weight above 25g health mice 60, Were randomly divided into two groups： The hungry group 30, Before the experiment strict fasting to restrain water 30h ; Repletion group, Free feeding, drinking water. Then by anthrone colorimetric method for determination of mouse liver glycogen, Using Folin- Wu method for the determination of blood glucose in mice. Result： Hunger and satiety were compared with blood glucose and hepatic glycogen in mice showed significantly decreased or increased trend （ That hungry mice blood glucose and liver glycogen reduction, Sated mice blood glucose and liver glycogen increases ） , And the P〈0.05 has statistical significance. Conclusion： Repletion after liver glycogen increases, Hunger reduced liver glycogen; Blood glucose was significantly elevated after feeding, Blood glucose was significantly reduced, hunger.

关 键 词：饥饿 饱食 肝糖原 血糖 

分 类 号：Q593.1[生物学—生物化学]