酸性染料比色法测定乌头属不同药材中乌头生物碱含量  

Content Determination of Aconitine-type Alkaloids in Aconitum Different Medicinal Materials by Acid-dye Colormetry

作　　者：张敏敏[1] 蔡晓丹[1] 李耿[1] 肖日平[1] 阮永队 马春玲[1] 陈长青[1] 赖小平[1] 

机构地区：[1]广州中医药大学,广东广州510006 [2]塘厦医院,广东东莞518110

出　　处：《中医药导报》2012年第8期78-79,82,共3页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家重点基础研究发展计划(973计划)课题(2009CB522803);广东省科技计划项目(2009A030100014)

摘　　要：目的:测定毒性中药附子、川乌、草乌、雪上一枝蒿的乌头生物碱含量,对这4种药材的临床用量进行控制。方法:用酸性染料比色法,测定制附子、川乌、草乌中雪上一枝蒿的乌头生物碱含量。结果:附子中生物碱含量为0.2403 mg/g,川乌为1.0412 mg/g,草乌为2.1149 mg/g,雪上一枝蒿为0.6702 mg/g。结论:本实验中建立的酸性染料比色法测定乌头生物碱含量,可作为评价毒性药材质量的根据。Objective： To determine the content of aconitine-type alkaloids in prepared aconite root, radix aconite, radix aconite kusnezoffii and Radix Aconiti Brachypodi, and to control Clinical dosage. Method： Acid-dye colormetry was employed to determine the content of Aconitine-type Alkaloids in prepared aconite root, radix aconite, radix aconite kusnezoffii and Radix Aconiti Brachypodi. Results： Content of alkaloids in prepared aconite root is 0.2403 mg/g, 1.0412 mg/g in radix aconite, 2.1149 mg/g in radix aconite kusnezoffii and 0.6702 mg/g in Radix Aconiti Brachypodi. Conclusion： The method to determine content of Aconitine-type Alkaloids in Aconitum different medicinal materials by Acid-dye colormetry can be regarded as stan- dard to evaluate quality of medicinal materials.

关 键 词：酸性染料比色法 乌头属 生物碱 附子 川乌 草乌 雪上一枝蒿 

分 类 号：R284.1[医药卫生—中药学]