复方中药对断奶獭兔盲肠内环境及脾脏IFN-γ、IL-10 mRNA表达的影响  

Effects of dietary supplemental compound of Chinese herbal medicine on caecum inner circumstance and mRNA expression of IFN-γ and IL-10 in spleen in weaned rex rabbits

作　　者：赵泮峰[1] 任战军[1] 朱江江[1] 王磊[1] 邓永荣 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100 [2]杨凌惠丰兔业公司,陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2012年第8期21-27,共7页Journal of Northwest A&F University(Natural Science Edition)

基　　金：陕西省农业攻关项目(2010K01-16)

摘　　要：【目的】研究复方中药(cCHM)添加剂对断奶獭兔盲肠内环境及脾脏IFN-γ、IL-10mRNA表达的影响,探讨其对机体抗病能力的调控作用及适宜用量。【方法】选用(35±2)日龄断奶、体质量相近的獭兔144只,随机分为对照组和3个试验组,每组6个重复,每重复6只幼兔。对照组饲喂添加0.1g/kg杆菌肽锌的日粮(不含抗生素等其他药物),试验组分别饲喂添加复方中药5g/kg(cCHM1组)、10g/kg(cCHM2组)、20g/kg(cCHM3组)的日粮,60d后,取脾脏和盲肠样品,测定盲肠微生物活力和数量,检测IFN-γ、IL-10的相对表达量。【结果】与对照组相比,cCHM2组獭兔盲肠内容物氨氮(NH3-N)浓度降低21.62%,菌体蛋白(MCP)含量提高16.81%,差异显著(P<0.05)。复方中药抑制大肠杆菌的效果不及抗生素,但cCHM2组、cCHM3组乳酸杆菌数量较对照组分别提高18.31%和16.50%,差异显著(P<0.05)。复方中药提高了脾脏IFN-γ、IL-10mRNA的相对表达量,cCHM2组和cCHM3组均极显著高于对照组(P<0.01)。【结论】复方中药添加剂可改善断奶獭兔盲肠内环境,增强机体的免疫功能,有利于断奶獭兔的肠道健康;该试验条件下复方中药添加剂的适宜用量为10g/kg。【Objective】 The trial was designed to study the effects of dietary supplemental compound of Chinese herbal medicine（cCHM） on caecum inner circumstance and mRNA expression of IFN-γ and IL-10 in spleen in weaned rex rabbits.The regulatory effect of cCHM on disease resistance of weaned rex rabbits was probed,and the appropriate supplemental amount was investigated.【Method】 A total of one hundred and forty-four weaned rex rabbits with（35±2） days of age and similar body weight were randomly divided into the control group and 3 experimental groups,with 6 replicates in each group and 6 rabbits per replicate.The control group was fed diet supplemented with 0.1 g/kg zinc bacitracin,and the experimental groups with 5 g/kg（cCHM1 group）,10 g/kg（cCHM2 group） and 20 g/kg（cCHM3 group） cCHM,respectively.After 60 days,caecum and spleen were collected to measure the caecum microorganism vitality and quantity,and to detect mRNA relative expression of IFN-γ and IL-10 in spleen.【Result】 Compared with the control group,cCHM2 group significantly decreased ammonia nitrogen（NH3-N） concentration by 21.62% and increased microbial protein（MCP） by 16.81%（P0.05）.Although the result of inhibiting E.coli in the experimental groups was not better than the control group,both cCHM2 group and cCHM3 group increased the amount of Lactobacilli by 18.31% and 16.50%（P0.05）,respectively.cCHM improved mRNA relative expression in spleen,compared with the control group,both cCHM2 group and cCHM3 group increased mRNA relative expression of IFN-γ and IL-10（P0.01）.【Conclusion】 cCHM as dietary additive can improve weaned rex rabbits caecum inner circumstance and immune function,which is beneficial to intestinal health.The appropriate supplemental amount of compound Chinese herbal additive is 10 g/kg under this experimental condition.

关 键 词：复方中药 断奶獭兔 盲肠内环境 MRNA表达 

分 类 号：S853.74[农业科学—临床兽医学]