新生大鼠缺氧缺血性脑损伤药物干预的研究  

Intervention of NGF and Don-Shen Root in Hypoxic-Ischemic Encephalopathy of Newborn Rat

作　　者：王小阳[1] 张国珍[1] 朱长连[1] 杨静丽[1] 邢清河 程秀永[1] 张欣欣[1] 郭丽英[2] 周元聪[2] 

机构地区：[1]河南医科大学第三附属医院儿内科,河南郑州450052 [2]中国科学院上海生化所,上海200031

出　　处：《中国当代儿科杂志》2000年第4期263-266,共4页Chinese Journal of Contemporary Pediatrics

基　　金：河南省医学科学院基金!资助 ( 9612 0 0 0 5 2 )

摘　　要：目的　探讨神经生长因子 (NGF)和丹参对新生大鼠缺氧缺血性脑病 (HIE)的防治作用。方法　选用 7日龄新生大鼠 ,在制成HIE模型前后给予NGF和丹参腹腔注射 ,HIE后分期处死大鼠分别测定谷氨酸 (Glu) ,天门冬氨硫 (Asp) ,一氧化氮合酶 (NOS)和做病理检查。 结果　HIE组 (30min)Glu ,Asp和 (48h)NOS较正常对照组明显升高。Glu :(887.0± 145 .2 ) μg/g ,vs.(776 .5± 10 3.0 ) μg/g ,(P <0 .0 5 ) ;Asp :(2 78.6± 48.2 ) μg/g ,vs.(2 2 4.8± 2 7.0 ) μg/g ,(P <0 .0 1) ;NOS :(3.34 6± 0 .745 )U/ml,vs.(2 .195± 0 .6 15 )U/ml,(P <0 .0 1)。NGF和丹参可以抑制HIE后的Glu ,Asp和NOS升高 ,并可减轻脑组织病理改变。Glu :(6 94.9± 83.9) μg/g ,vs .(887.0± 145 .2 ) μg/g ,(P <0 .0 1) ;Asp :(2 0 6 .1± 2 5 .4) μg/g ,vs.(2 78.6± 48.2 ) μg/g ,(P <0 .0 1) ;NOS :(2 .0 41± 0 .35 7)U/ml,vs.(3.34 6± 0 .745 )U/ml,(P <0 .0 1)。结论　NGF和丹参对HIE有防治作用。Objective To investigate the preventive and therapeutic effects of nerve growth factor (NGF) and dan-Shen root on hypoxic-ischemic encephalopathy (HIE) in newborn rats. Methods NGF and dan-Shen root were injected into 7-day-old rats before and after the HIE models were made. Glutamate (Glu), aspartate (Asp) and nitric oxide synthesis (NOS) levels in brain tissues and pathological changes were examined after the hypoxic-ischemic insults. Results Both (Glu, Asp) in 30 min and NOS were significantly increased in HIE rats compared with the control group. The difference between them was markedly significant. Glu: ( 887.0 ± 145.2 ) μg/g, ( 776.5 ± 103.0 ) μg/g, (P< 0.05 ),Asp: ( 278.6 ± 48.2 ) μg/g, ( 224.8 ± 27.0 ) μg/g, (P< 0.01 ), NOS: ( 3.346 ± 0.745 ) U/ml, ( 2.195 ± 0.615 ) U/ml,(P< 0.01 ), respectively. Glu, Asp and NOS after HIE and the pathological changes were reduced by NGF and dan-shen root as well. Glu: ( 694.9 ± 83.9 ) μg/g, ( 887.0 ± 145.2 ) μg/g, (P< 0.01 ), Asp: ( 206.1 ± 25.4 ) μg/g, ( 278.6 ± 48.2 ), (P< 0.01 ), NOS: ( 2.041 ± 0.357 ) U/ml, ( 3.346 ± 0.745 ) U/ml, (P< 0.01 ). Conclusions NGF and dan-shen root have preventive and therapeutic effects on HIE.

关 键 词：缺氧缺血性脑病 神经生长因子 丹参 新生儿 治疗 

分 类 号：R722.1[医药卫生—儿科]