小柴胡汤及其分解剂对柯萨奇B_3m病毒感染乳鼠心肌保护及细胞免疫调节作用的研究  

Myocardial Protection and Immunoregulation of Minor Bupleurum Decoction and Its Decomposed Preparations on Coxsackie B_3m Viral Myocarditis in Mice

作　　者：王雪峰[1] 刘芳[1] 魏克伦[2] 段淑敏[3] 崔晓岱[4] 

机构地区：[1]辽宁中医学院附属医院,沈阳110032 [2]中国医科大学第二临床医院 [3]中国预防医学科学院病毒所 [4]首都儿科研究所免疫生化研究室

出　　处：《中国中西医结合杂志》2000年第8期599-602,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金!资助课题 (No .39670 869)

摘　　要：目的 :观察小柴胡汤及其分解剂Ⅰ号和分解剂Ⅱ号对心肌细胞的保护和对机体的免疫调节作用。方法 :以柯萨奇病毒B3亲心肌株 (CVB3m)腹腔注射诱导Balb/c乳鼠心肌炎模型 ,分别予以小柴胡汤、分解剂Ⅰ号及分解剂Ⅱ号 ,经灌胃后 ,于不同时间进行各组的NK细胞活性、T细胞亚群及心肌病理组织学检查。结果 :(1)小柴胡汤和分解剂Ⅱ号在病毒性心肌炎急性期均能明显提高NK细胞活性 ,有调节T细胞亚群功能 ,与病毒对照组比较有显著性差异 (P <0 0 5)。而分解剂Ⅰ号此作用不明显。 (2 ) 3种治疗药物对心肌免疫损伤均有明显保护作用 ,小柴胡汤作用明显优于分解Ⅰ号和分解剂Ⅱ号 (P <0 0 5)。而分解剂Ⅰ号和分解剂Ⅱ号比较未见显著性差异 (P >0 0 5)。结论 :小柴胡汤和分解剂Ⅱ号对病毒性心肌炎NK细胞活性和T细胞亚群具有双向调节作用 ,3种治疗药物对心肌的免疫损伤均有明显保护作用。To study the effect of Minor Bupleurum Decoction (MBD) and its decomposed preparations, decomposition 1 (DC 1) and decomposition 2 (DC 2) in myocardial protection and immunoregulation on coxsackie virus B 3 m induced myocarditis in mice. Methods: Murine model of experimental myocarditis caused by coxsackie virus B 3 m was developed in Balb/c mice, and were treated by MBD, DC 1 and DC 2. Natural killer (NK) cell activity, T lymphocyte subsets and histopathological change of myocardium were examined at various times after MBD, DC 1 and DC 2 were taken. Results: (1)MBD and DC 2 could increase the NK cell activity significantly, and also regulate the T lymphocyte subsets, while DC 1 did not have this kind of effect; (2) All MBD, DC 1 and DC 2 could protect myocardium obviously, the effect of MBD is better than those of its decomposed preparations. Conclusion: MBD and DC 2 had bi directional regulation on NK cell activity and T cell subsets, all the 3 groups could obviously protect myocardium.

关 键 词：小柴胡汤 柯萨奇病毒B3M 心肌炎 免疫调节 

分 类 号：R285[医药卫生—中药学]