β_2-AR基因多态性与婴幼儿毛细支气管炎的相关性研究  

Relationship between genetic polymorphisms of β2-adrenergic receptor and infants bronchiolitis

作　　者：张蕾[1] 张季红[1] 徐佩茹[1,2] 

机构地区：[1]新疆医科大学第一附属医院儿科,新疆乌鲁木齐830054 [2]新疆维吾尔自治区循证医学研究所,新疆乌鲁木齐830054

出　　处：《中国医药导报》2012年第23期10-13,共4页China Medical Herald

摘　　要：目的探讨β2-肾上腺素能受体(β2-AR)基因与婴幼儿毛细支气管炎之间的关系。方法以毛细支气管炎患儿(n=131)和正常对照儿童(n=110)全血DNA为模板,采用PCR的方法扩增β2-AR基因,以DNA直接测序法对目的基因多态性进行分析,采用BLAST将测序结果与NCBI基因库进行相似性序列比较。结果毛细支气管炎组与对照组β2-AR 16位点基因型分布及等位基因频率比较差异均有统计学意义(P<0.05),两组间β2-AR 27位点等位基因分布差异有统计学意义(P<0.05),β2-AR 27位点基因型分布差异无统计学意义(P>0.05)。不同性别儿童β2-AR基因多态性差异无统计学意义(P>0.05)。结论β2-AR基因多态性和与毛细支气管炎存在一定的有相关性。Objective To study the correlation between genetic polymorphisms of β2-adrenergic receptor （β2-AR） and in- fants bronchiolitis. Methods The total DNA in the infants of bronchiolitis （n = 131） and normal infants （n = 110） were used as templates, β2-AR gene was amplified by PCR, the gene was detected by DNA sequencing technology, the similari- ty of sequencing results and NCBI gene bank was detected by BLAST. Results There was statistically difference in the fre- quencies of genotypes and alleles of β2-AR 16 loci between the two groups （P 〈 0.05）. There was no statistically difference in the frequencies of genotypes of β2=AR 27 loci between the two groups （P 〉 0：05）, but there was statistically difference in the frequencies of alleles of β2-AR 27 loci between the two groups. Conclusion There are some correlation between ge- netic polymorphisms of β2-AR and infants bronchiolitis.

关 键 词：Β2肾上腺素能受体基因 毛细支气管炎 基因多态性 

分 类 号：R725.622.1[医药卫生—儿科]