应用计算机辅助精子分析系统检测镉对大鼠精子运动能力的影响  

Application of computer assisted sperm analysis(CASA) system to the detection of influence of cadmium on rat sperm motility

作　　者：徐莉春[1] 王沭沂[2] 杨小芳[2] 王心如[2] 

机构地区：[1]徐州医学院 [2]南京医科大学应用毒理重点实验室,210029

出　　处：《中华劳动卫生职业病杂志》2000年第4期223-225,共3页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家自然科学基金!(39640 006)资助项目

摘　　要：目的　为镉的雄性生殖毒性评价筛选出敏感指标 ,以便对其生殖毒性进行早期监测。方法　应用氯化镉对成年雄性SD大鼠进行腹腔染毒 (连续 7d) ,染毒剂量分别为 0 .2、0 .4、0 .8mg/kg体重 (以镉计 )。对大鼠进行睾丸精子头计数 ,并计算每日精子生成量。应用计算机辅助精子分析(CASA)系统对附睾尾精子运动能力进行检测。结果　 0 .8mg/kg染镉组大鼠睾丸精子头计数和每日精子生成量为 (16 .45± 3 .0 1) 10 6/g和(2 .6 5± 0 .5 1) 10 6·d-1·g-1,比对照组 [(119.2 0± 14.5 1) 10 6/g和(19.5 1± 2 .37) 10 6·d-1·g-1]明显降低 ,0 .2mg/kg染镉组大鼠附睾尾精子曲线运动速度 (VCL)即明显降低 ,0 .4mg/kg染镉组大鼠精子运动能力的改变更为显著 ,差异均有显著性 (P <0 .0 5或P <0 .0 1)。 0 .8mg/kg染镉组未见活动精子。结论　精子运动参数是镉雄性生殖毒性评价较敏感的观察指标 。Objective To select sensitive index for early detection of the toxic effects of cadmium on male reproduction.Methods Different doses of cadmium chloride(0.2,0.4,0.8 mg Cd/kg body weight)were administrated ip to the adult male Sprague Dawley rats.Control animals received the same volume of 0.9% NaCl solution.After 7 days,the rats were sacrificed and the testes were removed from the animals.The number of testicular sperm head were counted and daily sperm production was calculated.The motility of the sperm obtained from cauda epididymides using the“diffusion”method was measured by computer assisted sperm analysis(CASA).Results The sperm head counts and daily sperm production decreased significantly in 0.8 mg/kg of Cd group.Obvious changes of motility of sperm were observed when cadmium could not affect daily sperm production in the low and middle dose groups.The motility of sperm in 0.4 mg/kg Cd group was reduced more significantly than in 0.2 mg/kg Cd group in which only VCL decreased markedly.In 0.8 mg/kg Cd group,no viable sperm was found. Conclusion Motility of sperm is a sensitive index to assess the toxicity of cadmium on male reproduction,and CASA is a powerful tool for the study of male reproductive toxicology.

关 键 词：镉 精子头计数 计算机辅助精子分析 精子运动 

分 类 号：R114[医药卫生—卫生毒理学]