赤土茯苓中总黄酮的含量测定  

Determination of General Flavonoids in Smilax Glabra

作　　者：李玉琪[1] 李玉莲 张莉 施建飞 姜维 麦军利 张克锦[2] 

机构地区：[1]中国人民解放军兰州军区乌鲁木齐总医院,新疆乌鲁木齐830000 [2]新疆医科大学药理教研室,新疆乌鲁木齐830054

出　　处：《解放军药学学报》2000年第4期211-213,共3页Pharmaceutical Journal of Chinese People's Liberation Army

基　　金：兰州军区医药卫生科研项目!LXH99-20;新疆军区医药卫生科研课题!No.99006

摘　　要：目的 :测定赤土茯苓中总黄酮含量。方法 :6 0 %乙醇提取液 ,三氯化铝醋酸钾显色 ,42 0nm测定紫外吸收度。结果 :12 .5- 2 5倍量 6 0 %乙醇回流提取 1h ,提取一次 ,总黄酮含量为 0 .2 6 % ;8倍量 6 0 %乙醇回流提取 1h ,提取 4次 ,总黄酮含量为 0 .4%。结论 :赤土茯苓中总黄酮含量为 0 .4%。本法简便、快速 。Aim To determine general flavonoids (GF) in smilax glabra.Methods The substances were extracted with 60% alcohol, developed with aluminum trichloride and potassium acetate, and determined at 420nm by UV spectroscopy.Results Smilax glabra was refluxed and extracted for one hour per time with 60% alcohol as much as 12.5～25 times the weight of the medicinal materials for one time. GF from smilax glabra was 0.26%. Smilax glabra was refluxed and extracted for one hour per time with 60% alcohol as much as 8 times the weight of the medicinal materials for four times.GF from smilax glabra was 0.4%.Conclusion The method could be used to determined GF in smilax glabra and control the quantity of the medicinal materials in its pharmaceutical preparations.

关 键 词：赤土茯苓 总黄酮 含量测定 紫外分光光度法 

分 类 号：R274.1[医药卫生—中医骨伤科学] R284.71[医药卫生—中医学]