基于HPLC-UV的代谢组学方法对六味地黄丸的抗炎药效评价  

Estimation of effects of Liuwei Dihuang Wan on anti-inflammation in rat by HPLC-UV based metabonomic method

作　　者：谢宝刚[1] 方修忠[2] 陈颖[1] 钟琳[1] 钟海军[1] 

机构地区：[1]南昌大学药学系,江西南昌330006 [2]南昌大学理学院,江西南昌330006

出　　处：《中国中药杂志》2012年第17期2635-2638,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30960456);江西省教育厅科技计划项目(GJJ09451)

摘　　要：目的:了解能否借助已建立的基于HPLC-UV的代谢组学技术对六味地黄丸的抗炎药效作用进行分析评价。方法:在提取获得六味地黄丸亲水或亲脂成分物质组的基础上,采用测定角叉菜胶致炎大鼠后右爪肿胀度及基于HPLC-UV的代谢组学方法对这两物质组的抗炎药效作用进行评价。结果:高剂量组对大鼠足肿胀有一定的抑制作用,差异明显;低剂量组与炎症组间没有统计学差异。而代谢组学分析显示大鼠给予低剂量的2种提取液后,其尿液HPLC-UV指纹图谱特征在主成分分析得分图上具有明显的回归趋势,其中亲水成分组尤为明显。结论:本研究结果表明基于HPLC-UV的代谢组学方法可用于六味地黄丸的抗炎药效评价,具有灵敏、整体等优点。Objective： To understand the possibility of estimating the anti-inflammation effect of Liuwei Dihuang Wan （LWW） in rat by having developed HPLC-UV metabonomic technology. Method： The hydrophilic or lipophilic constituent group of LWW was extracted by distilled water or acetic ether respectively.The anti-inflammation effects of different LWW dosages and extractions were measured by traditional method respectively.Then,metabonomic analysis was performed. Result： The high dosage of LWW extraction （16.5 g·kg-1）could inhibit the swell of rat palm significantly,but there are not statistically significant effect of low dosage group.Nevertheless,the metabonomic study showed that LWW extraction could restore obviously the rat HPLC-UV urinary profiling disturbed by inflammation in low dosage,especially the hydrophilic constituent group. Conclusion： Our study indicated that the developed metabonomic technology based on HPLC-UV might be used as a potentially powerful tool for estimating the anti-inflammation effect of LWW with sensitivity and integrity.

关 键 词：六味地黄丸 亲水或亲脂成分组 代谢组学 抗炎药效 

分 类 号：R285.5[医药卫生—中药学]